郎格罕氏组织细胞增生症的电镜研究  

Electron microscopic studies on Langerhans-cell histiocytosis

作　　者：郑晓刚[1] 陈旭东[1] 姜少军[1] 陆珍凤[1] 周晓军[1] 

机构地区：[1]南京军区南京总医院病理科,江苏南京210002

出　　处：《电子显微学报》2006年第3期250-253,共4页Journal of Chinese Electron Microscopy Society

摘　　要：目的:探讨郎格罕氏组织细胞增生症(LCH)的超微病理特点,提供Birbeck颗粒来源的线索。方法:结合光镜、免疫组化及临床资料,对2例LCH进行了超微结构观察。结果:朗格罕组织细胞胞浆内可见棒状、网球拍状Birbeck颗粒,这些细胞免疫组化CD1a阳性。结论:LCH的诊断须见到典型的郎格罕氏细胞(Langerhans cell,LC)LC细胞,电镜下找到Birbeck颗粒,或CD1a(O10)阳性。Birbeck颗粒可与细胞膜相连续,细胞膜凹陷可在胞质内形成Birbeck颗粒。Objective： To study ultrastructural features of Langerhans-cell histiocytosis（LCH） and provide some ultrastructural evidence to clarify the origin of Birbeck granules. Method： 2 cases of LCH were selected randomly. Specimens were treated with routine LM and TEM procedures, immunohistochemical method, and examined by light and electron microscope. Results： The Langerhans＇ cells showed positive immunostain for CDIa and S-100 protein. Under the electron microscope, ultrastructure of the Langerhans＇ cells revealed typical intracytoplasmic tennnis racquet-shaped structure with a central zipper-like striation （Birbeck＇s granules）. Conclusion： Birbeck＇s granules can serve as a useful marker for the identification of Langerhans-cell histiocytosis. The CDla and S-100 protein are demonstrated by immunohistochemical method, can serve this purpose as well. The development of Birbeck＇s Langerhans-cell granules is in evidence by invagination of the cell membrane ultrastructually.

关 键 词：郎格罕氏组织细胞增生症 电镜 Birbeck颗粒 

分 类 号：R551[医药卫生—血液循环系统疾病]