支气管哮喘患者对三种蟑螂变应原的特异性血清学反应  

The specific IgE responses to three cockroach strains antigens in patients with asthma

作　　者：郭胤仕[1] 许以平[1] 

机构地区：[1]上海交通大学医学院附属仁济医院呼吸科,200001

出　　处：《中华内科杂志》2006年第7期556-558,共3页Chinese Journal of Internal Medicine

基　　金：上海市高等学校科技基金资助项目(98B14)

摘　　要：目的探讨支气管哮喘患者对常见蟑螂的过敏水平及不同品种蟑螂间可能存在的交叉抗原性及其程度。方法分别提取美洲大蠊、黑胸大蠊和德国小蠊抗原,用ELISA法测支气管哮喘患者血清中三种蟑螂抗原的特异性IgE抗体(sIgE)。结果美洲大蠊、黑胸大蠊和德国小蠊sIgE阳性率分别为23．5％、16．0％和14．0％。美洲大蠊与黑胸大蠊、德国小蠊间血清学反应符合率分别为74．0％和73．5％,黑胸大蠊与德国小蠊间血清学反应符合率为85．0％。美洲大蠊头、体部抗原sIgE阳性率分别为17．5％和14．5％,头体部抗原间血清学反应符合率为86。0％;黑胸大蠊头、体部抗原sIgE阳性率分别为12．5％和10．5％,头体部抗原间血清学反应符合率为88．0％;ELISA交叉抑制实验显示,三种蟑螂相互之间均存在较明显的交叉抑制现象。结论蟑螂是诱发支气管哮喘的主要变应原之一。三种蟑螂之间及美洲大蠊、黑胸大蠊不同部位之间均存在一定程度的交叉抗原性成分,即具有共同的IgE结合组分。Objective The purpose of the present study was to determine the cross-reactive allergens from three strains of cockroach （Periplaneta americana, Periplaneta fuliginosa and Blattella germanica） by human serum specific IgE （sIgE） in allergic asthma. Methods The allergens were extracted from Periplaneta americana, Periplaneta fuliginosa and Blattella germanica respectively. Serum sIgE of allergic asthma was determined with ELISA. Results The sIgE positive rates to Periplaneta americana, Periplanetafuliginosa and Blattella germanica were 23.5%, 16. 0% and 14.0% respectively. The reactive coincidence rate between Periplaneta americana and Periplaneta fuliginosa was 74.0%. The reactive coincidence rate between Periplaneta americana and Blattella germanica was 73.5%. The reactive coincidence rate between Periplanetafuliginosa and Blattella germanica was 85.0%. The sIgE positive rates of the head and the body of Periplaneta americana were 17.5% and 14. 5% respectively. The sIgE positive rates of the head and the body of Periplanetafuliginosa were 12. 5% and 10. 5% respectively. The reactive coincidence rate between head and body of Periplaneta americana was 86.0%. The reactive coincidence rate between head and body of Periplaneta fuliginosa was 88.0%. There were some cross-inhibition among the three strains of cockroach according to ELISA cross-inhibition test. Conclusion Cockroach is one of the main allergens which cause allergic asthma. There exists significant cross-reactive allergic components in the three strains of cockroach and between the head and the body of Periplaneta fuliginosa and Periplaneta americana.

关 键 词：哮喘 变应原 特异性IGE 蟑螂 交叉反应抗原 

分 类 号：R562.25[医药卫生—呼吸系统]