光照、琼脂和碳源对离体培养中水曲柳根尖生长的影响  

Influence of light,agar and carbon sources to growth of in vitro cultured root tips of Fraxinus mandshurica

作　　者：冯丹丹[1] 王红梅[1] 张丽杰[1] 夏祥友[1] 沈海龙[1] 

机构地区：[1]东北林业大学林学院,哈尔滨150040

出　　处：《植物研究》2006年第4期424-426,共3页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(30170772)

摘　　要：研究了光照有无、琼脂添加与否和不同碳源对离体培养的水曲柳根尖生长的影响。结果表明,不添加琼脂的液体培养基对根尖的生长较添加琼脂的固体培养基和固—液培养基好,原因是增加琼脂的量会抑制根的生长;暗培养比光培养更有利于水曲柳离体根尖的培养;蔗糖作为碳源的效果较果糖和麦芽糖好,其中以3%的蔗糖对离体根尖的生长效果最好。The effects of light, agar and carbon sources on the of in vitro cultured root tips of Fraxinus mandshurica were studied. The results showed that the growth of root tips in liquid medium was better than that in agar-solidified medium and solid-liquid two phase medium, for that increasing agar concentrations in medium will reduce the root growth; darkness was beneficial for Fraxinus mandshurica root tip culture in vitro; as the carbon sources, sucrose was better than glucose and maltose as the carbon sources, and 3% sucrose was best for the root tip growth of Fraxinus mandshurica.

关 键 词：水曲柳 根尖 离体培养 

分 类 号：S792.41[农业科学—林木遗传育种] X511.032.31[农业科学—林学]