离体条件下几种不同的因子对芦笋诱导生根的研究  

Different factors on formation of rooting in Asparagua officinalis in vitro

作　　者：吴翠蓉[1] 刘丽娜[1] 于爱萍[1] 赵小梅[1] 周根余[1] 

机构地区：[1]上海师范大学生命与环境科学学院生物系遗传学实验室,上海200234

出　　处：《植物研究》2006年第4期430-434,共5页Bulletin of Botanical Research

基　　金：上海市高等学校科学技术发展基金项目(03DZ02)

摘　　要：以芦笋的茎尖为外植体,分别从糖、无机盐、激素等方面研究了在离体条件下影响芦笋生根的因素。结果分析表明:1/2MS、20g·L-1的糖有利于生根;10g·L-1糖、1/4MS培养基上预处理12d后,生根率可达到100%,根的质量明显优于生长在含有激素培养基上的根;激素预处理并不能提高生根率。因此,低盐及低糖预处理是提高芦笋生根率的一种比较好的方法。Using Asparagua officinalis stem shot as explant, the paper discussed the factors that affected the rooting in vitro. The concentration of sucrose, abiosalt and plant growth regulator were studied. The result indicated that 1/2 MS and 20 g·L^-1 sucrose promoted rooting;Rooting rate arrived at 100% when it was pretreated in the twelfth with 1/4 MS and 20 g·L^-1 sucrose. Its quality of root excelled the root in the medium with hormone;pretreatment of hormone couldn＇ t enhance rooting rate. Therefore,low salt or low sucrose pretreatment was a kind of good way for advancing rate of rooting in Asparagua officinalis.

关 键 词：芦笋 糖 无机盐 生根 

分 类 号：S792.99[农业科学—林木遗传育种] TS251.52[农业科学—林学]