应用原位杂交技术对小麦—黑麦代换系间杂交的细胞遗传研究  

Hybrids of wheat-rye substitution lines 5A/5R×6A/6R by genome in situ hybridization

作　　者：钟莉[1] 

机构地区：[1]浙江工业大学生物与环境工程学院,杭州310014

出　　处：《植物研究》2006年第4期442-446,共5页Bulletin of Botanical Research

摘　　要：应用原位杂交技术结合染色体组型分析方法,对两个小麦-黑麦异源双代换系5R/5A和6R/6A杂交后代的遗传进行了研究,探讨同祖染色体配对的可能性并获得小麦-黑麦易位系。实验中对杂种F1代植株减数分裂各时期的花粉母细胞染色体行为进行分析,结果发现有22·91%的花粉母细胞中黑麦染色体与小麦染色体发生同祖配对。F2代通过C-分带、原位杂交鉴定,在45株中检测到9株易位,易位频率为20%,是目前报道易位频率最高的。染色体易位有的来源于同祖配对交换,有的来源于单价体错分裂或断裂的重建。Genome in situ hybridization and C-banding were applied to study the hybrids of two wheatrye substitution lines 5A/SR and 6A/6R , the mechanism of homoeologous chromosome pairing was clarified and wheat-rye translocation lines was created. The chromosome behavior of pollen mother cell （PMCs） in meiosis was investigated in hybrid F1, homeologous chromosome pairing between wheat and rye occurred in 22.9% of PMC. Wheat-rye translocation lines were indentified via C-banding and Genome in situ hybridization （GISH） in hybrid F2 and later generations. In F2 generation, translocations happened in 9 of 45 plants, reached to 20%. These translocation lines were generated from homeologous chromosome pairing, or mis-division and reconstruction of univalent chromosomes.

关 键 词：代换系 易位系 减数分裂 原位杂交 同祖配对 

分 类 号：S947.2[农业科学—水产养殖] S512.1[农业科学—水产科学]