转MDMV CP基因玉米植株的再生  

Regeneration of transgenic maize plants with MDMV CP gene

作　　者：周小梅[1] 贾炜珑[2] 赵云云[3] 

机构地区：[1]山西大学生命科学与技术学院,太原030006 [2]天津大学化学系,天津300072 [3]首都师范大学生物系,北京100037

出　　处：《植物研究》2006年第4期461-464,共4页Bulletin of Botanical Research

基　　金：北京市教育委员会科技发展计划项目(01KJ-112)

摘　　要：用基因枪法将玉米矮花叶病毒外壳蛋白基因导入玉米自交系综31幼胚诱导的愈伤组织中,在含有Bialaphos6mg·L-1的选择培养基上经过3个月的抗性筛选,抗性愈伤组织在分化培养基上生成可育再生植株。PCR、PCR-Southern blot及DNA点杂交结果表明,外源基因已导入到玉米基因组中。转基因T1和T2代植株在大田表现出对MDMV的抗性,可以降低发病率,减轻发病程度。The maize calli derived from immature of inbred line Z31 were used for transformation mediated by microprojectile bombardment. The fertile transformants were obtained from resistant calli which had been subcuhured on selecting medium containing 6 mg·L^-1 Bialaphos for 3 months. Successful genetic transformation was confirmed by PCR , PCR-Southern blot assay and DNA dot hybridization. The incidence of virus disease was low on transgenic plants, thus showing that it had a certain resistance to MDMV.

关 键 词：玉米 外壳蛋白基因 转基因植株 基因枪法 

分 类 号：S435.131.4[农业科学—农业昆虫与害虫防治] S513.01[农业科学—植物保护]