酞酸酯对龙须菜的生态毒理研究  

Study on the ecological toxicology of PAEs to seaweed gracilaria lemanei formis

作　　者：吴志辉[1] 杨宇峰[1] 聂湘平[1] 李桂英[2] 李凯彬[3] 

机构地区：[1]暨南大学水生生物研究所,广东广州510632 [2]中国科学院广州地球化学研究所,广东广州510640 [3]中国水产科学院珠江水产研究所,广东广州510380

出　　处：《海洋科学》2006年第6期46-50,共5页Marine Sciences

基　　金：国家自然科学基金资助项目(4047304640471118);有机地球化学国家重点实验室开放基金资助项目(OGL-200306);国家973计划资助项目(2002GB412405)

摘　　要：研究了酞酸酯类化合物对龙须菜（Gracilaria lemaneiformis）生长的影响以及酞酸酯在龙须菜体内的积累状况。结果表明，在邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酯二异辛酯（DEHP）4种酞酸酯混合质量浓度为0．05，0．10，0．20和0．40mg／L的暴露条件下，龙须菜的相对生长速率和叶绿素a含量均受到了明显影响。与对照组比较，在高质量浓度处理组（0．1～0．4mg／L）中，两指标分别下降了18．4％～21.3％和10．4％～15．3％，致毒效应与酞酸酯暴露浓度呈正相关。在受控水体的暴露试验中，利用气相色谱（GC—FID）分析表明，4种酞酸酯类（DMP，DEP，DBP和DEHP）在龙须菜体内均有一定程度的积累，其残留量分别为0～0．14mg／kg，0～0．83mg／kg，0．11～1189mg／kg，0．01～10．4mg／kg，其积累程度与酞酸酯分子结构相关，侧链较长的酞酸酯比侧链较短的积累量要大，并随处理浓度的增加和时间推移呈增加趋势。The effects of phthalate esters （PAEs） on the growth of Gracilaria lernaneiformis, as well as the accumulation of PAEs in the seaweed, were studied by a series of indoor experiments. Results showed that the relative growth rate（RGR） and the chlorophyll a（Chl a） content of G. lernaneiforrnis were notably affected when exposed to the mixture of Dimethyl phthalate （DMP）, Diethyl phthlate（DEP）, Dibutyl phthlate （DBP） and Di（2-ethlhexyl） phthlate （DEHP） at the concentrations of 0.05, 0. 10, 0.20, 0.40 mg/L and the toxic effect of PAEs on the seaweed increased with the increasing of concentrations of PAEs. When the concentra- tion of PAEs was 0.1-0.4 mg/L, the RGR and Chl a contents decreased by 18.4%-21.3% and 10.4%-15.3%, respectively, as compared to control treatment. Analysis by means of gas chromatography（GC） showed that these four PAEs were accumulated in the seaweed to some extent, and the residues of DMP, DEP, DBP and DEHP were 0-0. 14 mg/kg, 0.23-0.83 mg/kg,0-l. 32 mg/kg and 0.38-9.72 mg/kg, respectively. Results indicated that the molecular structure of PAEs had a significant relationship with the accumulation of PAEs in sea algae, longer side-chain PAEs were accumulated more in G. lernaneiforrnis than the shorter ones did, and the amounts of these PAEs increased with increasing the exposed concentrations and time.

关 键 词：酞酸酯 龙须菜(Gracilaria lemaneiformis) 致毒效应 积累 

分 类 号：Q51[生物学—生物化学]