川芎嗪对AngⅡ诱导的VSMCs增殖中CaM和CaN活性的影响  

Effects of Tetramethylpyrazine on the Proliferation of Vascular Smooth Muscle Cells

作　　者：王梅娟[1] 郑红花[1] 罗德生[1] 李映红[1] 

机构地区：[1]咸宁学院医学院生物化学与分子生物学系,湖北咸宁437100

出　　处：《咸宁学院学报（医学版）》2006年第3期193-196,共4页Journal of Xianning Univarsity(medical Sciences)

基　　金：湖北省教育厅重点科研基金资助项目(2003A006)

摘　　要：目的探讨不同浓度川芎嗪在不同作用时间内对血管紧张素Ⅱ(AngⅡ)诱导的VSMCs(VSMCs)增殖的抑制作用。方法建立AngⅡ诱导VSMCs增殖模型,用酶促反应定磷法观察不同浓度的川芎嗪在不同时段内对VSMCs中钙调蛋白(CaM)和钙调神经磷酸酶(CaN)活性影响。结果成功建立AngⅡ诱导的VSMCs增殖模型,川芎嗪各组CaM和CaN活性随川芎嗪的浓度和作用时间的延长而显著下降(P<0.01)。结论川芎嗪对AngⅡ诱导的VSMCs增殖的抑制作用有剂量和时间依赖性,其抑制机制可能与其干预CaN依赖的信号转导途径有关。Objective To study the effects of tetramethylpyrazine（TMP） on calmodulin（CaM） and calcinuerin（CaN） in the proliferation of vascullar smooth muscle cells（VSMCs） induced by AngⅡ. Methods A cells proliferating model of VSMCs induced by angiotensinⅡ （AngⅡ ） was established; the effects of tetramethylpyrazine（TMP） on CaM and CaN were detected by enzyme reaction phosphorus measurement. Results Cell count, cell proliferation activity, CaM and CaN activities in VSMCs were obviously reduced as compared with Ang II group in a dose and time-dependent manner while treated with TMP（ P 〈0.01 ）. Conclusion The VSMCs proliferation induced by AngII can be inhibited by TMP in a dose and time-dependent manner and the inhibiting mechanism may be related to inhibiting CaM and CaN activities.

关 键 词：川芎嗪 VSMCS 钙调蛋白 钙调神经磷酸酶 

分 类 号：R963[医药卫生—微生物与生化药学]