体外受精周期中来源于单原核受精卵胚胎性染色体分析  

Sex chromosome analysis of embryos from monopronucleus zygotes derived from in-vitro fertilization and intracytoplasmic sperm injection in human

作　　者：颜军昊[1] 陈子江[1] 李媛[1] 胡京美[1] 高姗姗[1] 

机构地区：[1]山东大学山东省立医院生殖医学中心,山东济南250021

出　　处：《山东大学学报（医学版）》2006年第6期545-548,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30470703);山东省优秀中青年科学家科研奖励基金资助课题(03BS150)

摘　　要：目的:分析常规体外受精和单精子显微注射周期中来源于单原核受精卵胚胎性染色体的异常率。方法:应用FITCT、exas red标记的X/Y染色体着丝粒部位α卫星重复序列探针,对常规体外受精和单精子显微注射周期中来源于单原核受精卵的胚胎进行荧光原位杂交(fluorescent in situ hybridization,FISH),分析其性染色体的异常率和性染色体类型。结果:共分析来自常规体外受精和单精子显微注射周期中来源于单原核受精卵的胚胎111个。总胚胎固定率为92.80%,胚胎杂交率为95.15%。有杂交信号的常规体外受精后单原核受精卵发育来的胚胎中,胚胎性染色体二体率为54.35%,单体率为23.91%,嵌合体率为21.74%。有杂交信号的单精子显微注射后单原核受精卵发育来的胚胎中,胚胎性染色体二体率为34.62%,单体率为34.62%,嵌合体率为30.77%。结论:常规体外受精和单精子显微注射周期中来源于单原核受精卵的胚胎有高于正常的性染色体异常率,不利于进行胚胎移植;前者性染色体二体率显著高于后者。Objective： To examine the sex chromosomal aneuploid of embryos from monopronucleus zygotes derived from in-vitro fertilization （IVF） and intmcytoplasmic sperm injection （ICSI）. Methods： A total of 111 embryos from monopronucleus （1PN）, zygotes derived from IVF and ICSI were examined by using fluorescent in situ hybridization （FISH） technique with X, Y dual color centromere probes. Result： The total ratio of fixation was 92.80%, and of hybridization was 95.15%. In embryos from monopronucleus zygotes after IVF, the ratios of sex chromosome disome, monosomic and mosaic were 54.35 % ,23.91% and 21.74% ; in embryos from monopronucleus zygotes after ICSI, they were 34.62% ,34.62%and 30.77%. Conclusion： Embryos from monopronucleus zygotes after IVF or ICSI are not suggested to be transferred for the aneuploid ratio. Sex chromosome disome ratio of embryos from monopronucleus zygotes after IVF is significantly higher than that after ICSI.

关 键 词：非整倍体 原位杂交 荧光 胚胎 单原核 

分 类 号：R714.5[医药卫生—妇产科学]