瑞香素对恶性疟原虫细胞色素C氧化酶及核糖核酸还原酶活性的影响  

In Vitro Effect of Daphnetin on Cytochrome C Oxidase and Ribonucleotide Reductase of Plasmodium falciparum

作　　者：黄芳[1] 汤林华[1] 陈博[2] 倪奕昌[1] 王琴美[1] 

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾,血吸虫病和丝虫病合作中心,上海200025 [2]复旦大学生命科学学院遗传系,上海200433

出　　处：《中国寄生虫学与寄生虫病杂志》2006年第3期179-182,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的体外测定瑞香素对恶性疟原虫细胞色素C氧化酶(COX)及核糖核酸还原酶(RNR)活性的影响。方法Trager&Jensen法体外培养恶性疟原虫FCC1/HN分离株,超声波破碎恶性疟原虫提取总蛋白,用紫外分光光度计检测瑞香素与瑞香素-Fe复合物在不同作用时间和不同作用浓度对恶性疟原虫COX活性的影响,以电子自旋共振法检测经瑞香素与瑞香素-Fe复合物作用1、2、3和4h后,恶性疟原虫酪氨酸(Tyr)自由基的量以反映恶性疟原虫RNR的活性。结果体外同步培养的恶性疟原虫经瑞香素(100μmol/L)作用2、4、8和12h后,COX活性分别被抑制了0、6%、73%和80%;在瑞香素浓度为0.1、1、100和1000μmol/L,作用6h后,COX活性分别被抑制3%、31%、53%和84%;而瑞香素-Fe复合物对COX的影响几乎消失。经瑞香素(100μmol/L)作用1、2、3和4h后RNR活性分别被抑制7%、51%、69%和75%;在瑞香素浓度为0.1、1、100和1000μmol/L,作用6h后,RNR活性分别被抑制3%、31%、58%和93%;而瑞香素-Fe复合物作用6h后RNR活性分别被抑制8%、6%、11%和9%。结论在体外瑞香素可显著降低恶性疟原虫的细胞色素C氧化酶(COX)及核糖核酸还原酶(RNR)活性。Objective To test the in vitro effect of daphnetin on cytochrome C oxidase （COX） and ribonucleotide reductase （RNR） activity of Plasmodium falciparum. Methods P. falciparum （FCC1/HN） was cultured in vitro using the method of Trager and Jensen. The effect of daphnetin and daphnetin-Fe complex on COX and RNR activity of P.falciparum was tested by ultraviolet spectrophotometer and electron spin resonance （ESR） respectively. Result The parasites synchronized with sorbitol in vitro was treated by daphnetin and daphnetin-Fe complex. The inhibition level of the COX activity by daphnetin after being treated for 2, 4, 8 and 12 h were 0.6%, 73% and 80% respectively and the inhibition level by daphnetin at different concentrations （0.1, 1, 100 and lmmol/L） for 6h was 3%, 31%, 53% and 84%, respectively. No considerable effect was observed on the COX activity of P.falciparum treated with daphnetin-Fe complex. The tyrosyl free radical was tested to reflect the RNR activity of P.falciparum at various times by ESR. The inhibition level by daphnetin for 1, 2, 3 and 4 h were 7%, 51%, 69% and 75% respectively, while the values treated by daphnetin-Fe complex were 8%, 6%, 11% and 9% respectively. The inhibition level by daphnetin at different concentrations （0.1, 1, 100 and 1 mmol/L） for 6 h was 3%, 31%, 58% and 93% respectively and while the values treated by daphnetin-Fe complex were 8%, 6%, 11% and 9%. Conclusion Daphnetin significantly reduces the COX and RNR activities of Plasmodium falciparum in vitro.

关 键 词：瑞香素 恶性疟原虫 细胞色素C氧化酶 核糖核酸还原酶 

分 类 号：R382.312[医药卫生—医学寄生虫学]