再生障碍性贫血雄激素受体与T细胞亚群的变化  

The expression of androgen receptor and changes of T-cell subpopulations in chronic aplastic anemia

作　　者：张秀莲[1] 张伟华[1] 吴淑芬[1] 范星火[1] 李殿青[1] 

机构地区：[1]山西医科大学第一医院血液科,太原030001

出　　处：《临床血液学杂志》2006年第4期207-209,共3页Journal of Clinical Hematology

摘　　要：目的:检测慢性再生障碍性贫血(CAA)患者骨髓单个核细胞(MNC)雄激素受体(AR)以及骨髓中T细胞亚群水平,探讨AR在CAA免疫病理机制中的作用。方法:①免疫细胞化学SP法检测CAA患者骨髓MNC内AR的表达水平;②流式细胞术检测CAA骨髓中T细胞亚群(CD3+CD4+细胞、CD3+CD8+细胞)的含量。结果:①CAA患者骨髓MNC中AR阳性水平[(35．18±8．78)个／200个MNC]显著低于对照组[(48．46±9．82)个／200个MNC];不同性别间AR的含量差异无统计学意义。②CAA患者骨髓中的CD3+CD8+细胞含量为(28．54±7．57)％,显著高于对照组。③CAA患者骨髓中的AR阳性水平与骨髓中的CD3+CD8+细胞呈负相关(r=-0．576,P<0．01);而与CD3+CD4+细胞含量未见明显的直线相关关系。结论:CAA患者骨髓AR的表达减少,从而使雄激素刺激造血的作用减弱。患者AR的表达与CD3+CD8+细胞含量呈明显负相关,表明AR的异常可能在一定程度上参与了CAA发病机制中的细胞免疫。Objeetive..To study the effect of androgen receptor （AR） on immune pathogenesis of chronic aplastic anemia（CAA）. Method： ①lmmunocytochemistry was performed to evaluate the expression of AR in bone marrow mononuclear cells（MNCs） in 40 patients with chronic aplastic anemia. ②Flow cytometry was used to evaluate the contents of Tcell subpopulations（CD3^＋ CD4^＋ cells and CD3^＋ CD8^＋ cells） in bone marrow mononuclear cells. Result：The expression of AR in bone marrow MNCs of CAA patients was significantly lower than that of the con trol group; the contents of CD3^＋ CD8^＋ lymphocytes in CAA patients were significantly higher than that of the control group; In addition, the expression of AR in bone marrow had a negative relationship with CD3^＋ CD8^＋ lymphocytes in bone marrow. Condusion ：. The lower expression of AR in CAA patients induced the weaker stimulating of androgen to hematopoiesis in bone marrow. The AR expression in patients had a negative relationship with the contents of CD3^＋ CD8^＋ lymphocytes, which suggested the abnormality of AR might have an effect on cellular immune in pathogenesis of chronic aplastic anemia.

关 键 词：贫血 再生障碍性 慢性 雄激素受体 细胞免疫 

分 类 号：R556.5[医药卫生—血液循环系统疾病]