大菱鲆受精过程的细胞学观察  

Cytological observation on the fertilization process in Scophthalmus maximus L.

作　　者：吴莹莹[1] 柳学周[1] 王清印[1] 马爱军[1] 包振民[2] 

机构地区：[1]中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071 [2]中国海洋大学生命科学与技术学部,山东青岛266003

出　　处：《中国水产科学》2006年第4期555-560,共6页Journal of Fishery Sciences of China

基　　金：国家"863"高技术研究与发展项目(2002AA603011;2004AA603320);农业部农业结构调整重大技术研究专项项目(04-11-01B)

摘　　要：采用人工授精和组织切片技术对大菱鲆(Scophthalmus maximusL.)受精过程进行细胞学观察。大菱鲆成熟卵处于第二次成熟分裂的中期。精子入卵后,卵子被激动,第二次成熟分裂继续进行,同时发生皮层反应;受精后15 min出现精子星光;受精后20 min雄原核早于雌原核形成,然后两性原核逐渐靠拢;受精后30 min两原核相互靠拢,结合线清晰;受精后40 min两原核结合线逐渐消失联合成合子核,之后合子核核膜消失;受精后50 min合子核处于第一次有丝分裂中期;受精后60 min第一次卵裂完成。[中国水产科学,2006,13(4):555-560]This paper deals with the results of cytological observation on the fertilization process in turbot Scophthalmus maximus L. Artificial insemination and histological section techniques were used in this study. The fertilized eggs at different time after insemination were fixed in Bouin＇s solution and dehydrated by alcohol. The thickness of the tissue sections was from 6 to 8μm. Then the tissue sections were stained with hematoxylin and eosin. Observation results showed that the mature eggs of the Scophthalmus maximus L. remained at the metaphase of the second maturation division. The egg was activated as the sperm penetrating into the egg, with the development of the second maturation division and the cortex reaction. At 15 minutes after insemination, the sperm-aster appeared. At 20 minutes after insemination, the male pronucleus was formed prior to the formation of female pronucleus, and later they got closer to each other. At 30 minutes after insemination, the male and female pronuclei combined together and a clear junction line was observed. At 40 minutes after insemination, the zygote nucleus formed and later the karyotheca became faint. At 50 minutes after insemination, the zygote nucleus developed into the metaphase of the first mitosis, and then at 60 minutes, the first karyokinetic division was finished. From the results, it was concluded that the fertilization type of Scophthalmus maximus L. was monospermism and the combine type of male and female pronuclei was conjugation. [Journal of Fishery Sciences of China, 2006,13 （4） ：555 - 560 ]

关 键 词：大菱鲆 受精 细胞学 

分 类 号：S965.199[农业科学—水产养殖]