鹅源H5N1亚型禽流感病毒株HA基因的克隆与序列分析  

Cloning and Analysis of HA Gene of Goose H5N1 Influenza A Virus

作　　者：王伟利[1] 周宇[2] 孟庆锋[2] 刘明[3] 钱爱东[2] 

机构地区：[1]吉林出入境检验检疫局,吉林长春130062 [2]吉林农业大学动物科技学院,吉林长春130118 [3]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150001

出　　处：《中国兽医学报》2006年第4期385-386,389,共3页Chinese Journal of Veterinary Science

基　　金：吉林省重大科技专项基金资助项目(20040202-3-2)

摘　　要：本研究对1株鹅源H5N1亚型禽流感病毒血凝素（HA）基因进行了扩增、克隆及序列测定。结果表明：所扩增的片段长1707bp，包含完整的开放阅读框架，编码568个氨基酸。该毒株裂解位点的氨基酸序列为RRRKKR，具有高致病性的分子特征；该毒株有6个潜在糖基化位点；受体结合位点的氨基酸分别为YWIHELY，其左侧壁氨基酸为SGVSSA，右侧壁为NGQSGR。该毒株与国内外一些参考毒株的HA基因序列比较，核苷酸同源率为76.8％～98．1％，氨基酸同源率为85．7％～97．4％，属于欧亚种系。In this study,the eDNA of HA gene of a goose avian influenza virus isolate was amplified and cloned ,and then sequenced. The sequence analysis shows that eDNA of the HA gene is composed of 1 707 bp nucleotides containing whole open reading frame of HA gene and coding 568 amino acids. Amino acids sequence RRRKKR at the cleavage site is symbol of highly pathogenic avian influenza ;There are 6 potential glycosylation sites in the influenza virus isolate;Amino acids of the receptorbinding site are YWIHELY,amino acids of left edge of the receptor-binding site are SGVSSA,amino acids of right edge of the receptor-binding site are NGQSGR. The comparative sequence analysis indicated there was 76.8M-98. 1M of nueleotide homology and 85.70%-97.4% of amino acid homology with some reference strains. This goose isolate was recognized to be originated from Euroasian lineage.

关 键 词：鹅 禽流感病毒 血凝素基因 序列分析 

分 类 号：S852.65[农业科学—基础兽医学] S858.33[农业科学—兽医学]