实验性肉种鸡葡萄球菌关节炎的发病机制——左旋氧氟沙星治疗后鸡血清细胞因子水平的动态变化  

Pathogenesis of Experimental Staphylococcus Arthritis in Chickens: Dynamic Changes of IL-1β,IL-2 and TNF-α Levels in Serum After Therapy with Levofloxacin

作　　者：李秀梅[1] 谷长勤[1] 胡薛英[1] 周全[1] 周诗其[1] 程国富[1] 

机构地区：[1]华中农业大学动物医学院,湖北武汉430070

出　　处：《中国兽医学报》2006年第4期432-434,共3页Chinese Journal of Veterinary Science

基　　金：湖北省自然科学基金资助项目(2004ABA100)

摘　　要：5周龄健康艾维茵肉鸡120羽随机均分为空白对照组、药物对照组、感染组和药物治疗组。人工复制鸡葡萄球菌性关节炎的病理模型,并用左旋氧氟沙星治疗,测定血清中IL-1β、IL-2、TNF-α的动态变化。结果表明:整个试验过程中,感染组及感染治疗组血清中IL-1β、IL-2、TNF-α的水平与空白对照组相比,均有不同程度的升高;感染治疗组与感染组比较,血清中IL-1β的含量给药后1、7、14、21、28 d差异显著(P<0.05);血清中IL-2的含量7、14、21、28、35 d差异显著(P<0.05);血清中TNF-α的含量5、7、14、28 d差异显著(P<0.05)。由此认为上述3种细胞因子参与了鸡葡萄球菌关节炎的发生、发展,且IL-1β、TNF-α以损伤为主。One hundred and twenty 5-week-old healthy broilers were divided at random into four group：vacancy control group,drug control group,infection control group and test group, 30 every group. By replicating artificially the arthritis model with Staphylococcu.s aureus and therapy with levofloxacin,the dynamic changes of interleukin-1β（IL-1β）,interleukin-2（IL-2） and tumor necrosis faetor-α（TNF-α） levels in serum were measured. The result indicated that IL-1β,IL-2 and TNF-α levels in serum,all had a rise in different degree,in the infection control group and test group as compared with vacancy control group, and that between the infection control group and test group,the serum IL-1β levels had a significant differenee（P〈0. 05） at 1, 7,14,21,28 d after therapy with levofloxaein; the IL-2 at 7,14,21,28,35 d and TNF-α at 5,7,14,28 d did so. It was concluded that the three eytokines above mentined took part in the pathogenesis of arthritis in chicken.

关 键 词：鸡 葡萄球菌关节炎 左旋氧氟沙星 IL-1β IL-2 TNF—α 

分 类 号：S855.11[农业科学—临床兽医学] S858.31[农业科学—兽医学]