促卵泡激素和胰岛素对牛卵泡颗粒细胞长期培养的影响  

Effect of FSH and Insulin on Bovine Granulosa Cells in Long Term Serum-Cultrue

作　　者：高庆华[1] 周虚[1] 王春清[2] 韩春梅[3] 叶云鹏 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]吉林农业科技学院,吉林吉林市132109 [3]新疆塔里木农垦大学动物科技学院,新疆阿拉尔843300 [4]河北省昌黎县畜牧局,河北昌黎066000

出　　处：《中国兽医学报》2006年第4期442-443,447,共3页Chinese Journal of Veterinary Science

基　　金：吉林省杰出青年基金资助项目(20030121);吉林省科技发展计划项目(20010537)

摘　　要：以M cCoy′s5a为基础培养液添加硒、转铁蛋白、雄烯二酮、谷氨酰胺,对牛卵巢上大(直径>8 mm)、中(4 mm<直径<8 mm)、小(直径<4 mm)非闭锁卵泡的颗粒细胞进行无血清培养,研究添加不同浓度的促卵泡激素(FSH)和胰岛素对颗粒细胞增生、分化和激素分泌的影响。结果,FSH能诱导颗粒细胞增生及提高其雌二醇合成能力,颗粒细胞雌二醇的合成能力与生理浓度的FSH存在剂量依赖关系。胰岛素对大、中、小卵泡颗粒细胞增生和雌激素分泌有促进作用。A new mechanical method for bovine granulosa cells from different non-atretic follicles size categories （〈4 mm. 4-8 mm,and 〉 8 mm diameter） and a serum-free culture system using McCoy＇s5a medium （pH7.4） supplemented with 20 mmol/L Hepes ,3 mmol/L L-glutamine,0.1 % BSA, 29 μg/L testosterone,2.5 mg/L transferrin and 4 mg/L selenium for culture in vitro of bovine granulosa cells were developed. The results indicated that in physiological concentrations of FSH,granulosa cells from small follicles differentiated in vitro and secretion of estradiol increased with time（P〈0.01）. FSH and insulin stimulated proliferation of granulosa cells from follicles of different sizes,insignificantly. FSH and insulin stimulated estradiol secretion by granulosa cells from follicles of different sizes in a dose-responsive manner（P〈0. 01 ）.

关 键 词：牛 无血清培养 颗粒细胞 FSH 雌二醇 胰岛素 

分 类 号：S823[农业科学—畜牧学]