亚心形扁藻多糖的提取分离纯化及组成研究  

Study on the Extraction and Purification and Composition Analysis of Platymonas subcordiformis (Will) Hazen

作　　者：李亚清[1] 杨海波[2] 

机构地区：[1]大连理工大学精细化工国家重点实验室,辽宁大连116012 [2]大连大学化学与化学工程系,辽宁大连116622

出　　处：《微生物学杂志》2006年第3期20-22,共3页Journal of Microbiology

基　　金：辽宁省博士科研启动基金资助项目(001059)

摘　　要：以实验室培养的亚心型扁藻为原料,热水提取扁藻多糖。多糖经加热除蛋白、乙醇分级、Sephadex G-50柱层析等一系列纯化步骤后,得到一扁藻多糖(简称PPSc-1)。PPSc-1经醋酸纤维素薄膜电泳和SephadexG-100柱层析的纯度鉴定,组分均一。将多糖PPSc-1用三氟乙酸全水解,然后进行HPAEC-PAD分析,结果表明组成该多糖的单糖为D-半乳糖、D-2-氨基葡萄糖、D-阿拉伯糖、D-鼠李糖和3种未知单糖。各种已知单糖的摩尔比是D-半乳糖∶D-2-氨基葡萄糖∶D-阿拉伯糖∶D-鼠李糖=59.64∶40.9∶18∶1。Platymonas subcordiformis （ Will ） Hazen was cultured in order to extract intracellular polysaccharide using hot water. The free proteins were removed by heating from crude polysaccharides and then a kind of Platymonas subcordiformis （Will） Hazen polysaccharide （PPSc-1） was isolated and purified by ethanol precipitation, Sephadex G-50 gel chromatography. The composition of this polysaccharide was uniformed by means of cellulose film electrophoresis and Sephadex G-100 gel chromatography. PPSc-1 was analysed by HPAEC-PAD after hydrolyzed by trifluoroacetic acid. The result indicated that this polysaccharide was mainly composed of D-galactose, D-2-glu-osamine, D-arabinose, D-rhamnose and three unkown monosaccharides. Mole ratio of the four known monosaccharide is 59.64 ： 40.9：18 ： 1 correspondingly.

关 键 词：亚心形扁藻 多糖 组成 分离 纯化 

分 类 号：Q539[生物学—生物化学]