内生真菌感染对黑麦草抗盐性的影响  

Effect of endophyte infection on salt resistance of ryegrass

作　　者：任安芝[1] 高玉葆[1] 章瑾[1] 张晶[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《生态学报》2006年第6期1750-1757,共8页Acta Ecologica Sinica

基　　金：国家重点基础研究发展规划资助项目(G2000018601);国家自然科学基金资助项目(30370239);教育部科学技术研究重点资助项目(03046)~~

摘　　要：以感染内生真菌的多年生黑麦草(Lolium perenneL.)(SR4000)为实验材料,建植内生真菌感染(EI)和不感染(EF)的黑麦草种群,并对其进行盐胁迫实验,通过观察生长和生理生态指标的变化,分析内生真菌对宿主植物抗盐性的影响。结果表明,内生真菌感染对宿主黑麦草的营养生长没有增益效应,相反在高盐浓度下,EI种群的分蘖能力和地上部分生物量均低于EF种群;但内生真菌能够改变宿主种群生物量的分配格局,将更大比例的生物量分配于根系。在高盐浓度下,内生真菌感染可导致黑麦草叶内的脯氨酸含量显著增加、可溶性糖含量显著降低,但对PSⅡ光化学效率Fv/Fm值的变化没有影响。总体来看,内生真菌感染并未改善宿主黑麦草的抗盐性。In their evolution, grasses have developed symbiotic associations with fungi including mycorrhizal fungi that grow in or on the roots, and endophytes that live their entire life cycle within the aerial portion of the host grass, typically forming nonpathogenic, systemic and usually intercellular associations. Currently, seven genera have been identified, including Atkinsonella, Balansia, Balansiopsis, Echinodothis, Epichloe, Myriogenospora and Parepichloe. Among them, the anamorphic （imperfect） stage of Epichloe spp., i.e. Neotyphodium spp., is most closely related to cultivated grasses. The most widely known Neotyphodium endophytes are N. lolii and N. coenophialum, which colonize perennial ryegrass （ Lolium perenne L. ） and tall fescue （ Festuca arundinaceae Schreb. ） respectively. Endophytes may protect the hosts from biotic and abiotic stresses. In the past twenty years, much research has focused on the beneficial effect of endophyte infection on the biotic stress resistance of host grasses such as mammalian, insect and nematode herbivores. When abiotic stress was regarded, many studies showed that endophyte infection could improve drought resistance ability of host grasses. Similar to drought stress, salt stress also led plants to physiological drought. But up to now, no related research results were reported. Thus, in this paper Lolium perenne cv SR4000 infected by Neotyphodium lolii was chosen as experimental material. Vegetative growth and several physiological indexes （such as free proline content, soluble sugar content and photochemical efficiency of PSII） of endophyte-infected （EI） and endophyte-free （EF） populations under normal and salt stress conditions were compared in order to discuss the effect of endophyte infection on the salt resistant ability of the host ryegrass. The experiment was performed under greenhouse condition. EI and EF plants were transplanted into culture pots filled with 1L 1/2 Hoagland solution. One week later, salt （NaCl） was added to impose salt

关 键 词：黑麦草 内生真菌 盐胁迫 

分 类 号：S543.6[农业科学—作物学]