干旱与条锈病复合胁迫对小麦的生理影响  

Combined effect of water stress and pathogen infection on wheat physiology

作　　者：冯汉青[1] 吴强[1] 李红玉[1] 万东石[1] 贾秋珍[2] 李敏权[3] 梁厚果[1] 

机构地区：[1]兰州大学生命科学院,甘肃兰州730000 [2]甘肃省农科院植保所,甘肃兰州730070 [3]甘肃农业大学植保系,甘肃兰州730070

出　　处：《生态学报》2006年第6期1963-1974,共12页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(301702383和30471133)~~

摘　　要：以抗旱性和抗病性不同的小麦为材料,以正常生长为对照,观察了病原菌和水分复合胁迫对小麦叶片相对含水量、活性氧代谢以及对抗氰呼吸的发生、运行的影响。讨论了在干旱与病原菌侵染复合胁迫下,抗氰呼吸在植物抗逆机制中所扮演的角色。复合胁迫下,抗病小麦显然具备更强的水分调控能力,而感病品种不能有效控制病叶水分散失。水分胁迫能引起抗氰呼吸的下降,但不能抵消因病原菌侵染引起的抗氰呼吸的增强,条锈菌侵染对小麦抗氰呼吸的影响远远大于水分胁迫。病原菌侵染和水分复合胁迫下,活性氧产生的速率表现出累加效应,而抗氰呼吸表现出和基质抗氧化酶的活性互补。植物交替氧化酶在干旱与病原菌侵染复合胁迫中具有重要的抗氧化功能,并可能调节着逆境下物质与能量需求间的矛盾。Relative water content （RWC）, operation and development of alternative pathway, the changes in active oxygen species （AOS） and activities of antioxidant enzymes, and the role of alternative pathway participating in the protection mechanism against environment stress were studied and discussed in wheat seedlings leaves under combined intimidation of water stress and pathogen infection. Experiments showed that the disease-resistant cultivars had more powerful ability to regulate moisture content than the disease-susceptible cuhivars under combined intimidation of water stress and pathogen infection. The effects of stripe rust cyanide-resistant respiration were very larger than that of water stress. The decrease in cyanide-resistant respiration caused water stress couldn＇t counteract the increasing cyanide-resistant respiration aroused by stripe rust infecting. Combined intimidati resulted in AOS accumulation, and cyanide-resistant respiration and the activities of antioxidant enzymes presented upon t completion of lowing AOS production, to some extent. It was proposed that cyanide-resistant respiration participates in the antioxidation mechanisms and regulate the ambivalence and balance of requirement of the energy and substance for combine intimidation conditions.

关 键 词：小麦 条锈病 水分胁迫 复合胁迫 活性氧 抗氰呼吸 抗氧化酶 

分 类 号：S512.1[农业科学—作物学]