New Approach to Detecting Phenoxyl Free Radicals Generated in Enzyme Reaction by Stopped-flow Spectrophotometry  

作　　者：Jing XU Lan Hua ZHAO Xin Guo WU Hong Mei WANG Ru Xiu CAI 

机构地区：[1]College of Chemistry and Molecular Science, Wuhan University, Wuhan 430072 [2]Stuff of Chemistry, Yunyang Medical College, Shiyan 442000 [3]College of Resources and Environmental Science, Wuhan University, Wuhan 430072

出　　处：《Chinese Chemical Letters》2006年第7期949-952,共4页中国化学快报（英文版）

基　　金：The authors gratefully acknowledge financial support from the National Natural Science Foundation of China （No. 20275027 and No. 20377032）.

摘　　要：A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide （NADH）. Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9 mol/L.A highly sensitive stopped-flow spectrophotometry kinetic method was proposed for quantification phenoxyl radicals based on their accelerating effect on the oxidation of nicotinamide adenine dinucleotide （NADH）. Phenoxyl radicals generated from as low as 1×10^-8 mol/L 2,4-DCP can be readily detected with the proposed method and the detecting limit was 2.5×10^-9 mol/L.

关 键 词：Phenoxyl radicals moleculary probe stopped-flow spectrophotometry. 

分 类 号：O641.3[理学—物理化学]