偶联植物多糖对日本血吸虫疫苗PV1223增效作用及其机制的初步探讨  

Studies on Effect Enhancement of Vegetal Polysaccharide on Schistosoma Japonicum DNA Vaccine PV1223

作　　者：曹恒[1] 熊娟[1] 柳晶[1] 冯清[2] 甘燕[3] 石佑恩[3] 

机构地区：[1]华中科技大学同济医学院临床医学学生,武汉430030 [2]华中科技大学化学系室,武汉430030 [3]华中科技大学同济医学院寄生虫学教研室,武汉430030

出　　处：《中国药学杂志》2006年第12期899-901,共3页Chinese Pharmaceutical Journal

基　　金：湖北省重点科技攻关项目(2001AA301C31和2003AA301D13)

摘　　要：目的以天然提取香菇多糖和茶叶多糖混合物(茶叶多糖-香菇多糖=1∶1)为佐剂,探讨疫苗-佐剂偶联物对血吸虫疫苗的保护性免疫的增效作用及其机制。方法采用108CFU日本血吸虫疫苗PV1223与植物多糖偶联后皮下接种小鼠,同时设立对照组。接种后第8周以日本血吸虫尾蚴攻击感染,感染后6周剖杀小鼠,计算减虫率、减卵率,并测定血清中特异性抗体水平。结果实验组减虫率和减卵率分别达到65.96%,80.88%,与对照组相比有非常显著意义(P<0.01),而免疫前后各实验组IgG水平却无统计学差异。结论偶联植物多糖对日本血吸虫疫苗PV1223有明显的增效作用,其免疫机制可能以细胞免疫为主。OBJECTIVE To investigate the immunogenicity of schistosma japonicum DNA vaecine PV1223 enhanced by likage vegetal polysaccharide. METHODS BALB/c mice were immunized subcutaneously with 10s CFU PV1223 combined with tea and mushroom polysaccharides in linkage, and then challenged with schistosoma japonica cercariae on the 8th week after immunization,and killed on the 6th week of infection. The rate of reduced worm burdens, reduced egg burdens, and the examination of the IgG level were detemained. RESULTS The rates of reduced worm and egg burdens were 65.96% and 80.88%, respectively. There were remarkable difference between control and immunized group. But no significant differences among the IgG titres were observed after challenge. CONCLUSION This study indicales that the vegetal polysaccharide linked with vaceine PV1223 can promote the efficacy of vaccine PV1223 by the mechanism of cellular immune resporlae.

关 键 词：血吸虫病 偶联 植物多糖 疫苗佐剂 

分 类 号：R965[医药卫生—药理学]