人源抗D-二聚体抗体的构建和分析  

Construction and Charaterization of Hominine Anti-D-dimer Antibody

作　　者：田朝伟[1] 陈颖[2] 杨晓仪[2] 贾宗剑[2] 吴文言[2] 黄少华[1] 陈敏生[1] 

机构地区：[1]广州医学院第二附属医院急诊科,510260 [2]中山大学生命科学学院医药分子生物实验中心

出　　处：《岭南急诊医学杂志》2006年第3期180-181,共2页Lingnan Journal of Emergency Medicine

摘　　要：目的:从人源性噬菌体抗体库中筛选抗人D-二聚体单克隆抗体,构建一种导向溶栓抗体。方法:应用噬菌体展示技术构建血栓病人的抗体库,从中淘选出能与血栓主要成份纤维蛋白的降解产物D-二聚体有特异结合作用的抗体。将抗体在XL1-Blu中可溶性表达。再利用抗D-二聚体鼠源抗体进行竞争ELISA检验,证实抗体与D-二聚体结合的活性。结果:获得抗人D-二聚体单克隆抗体D13株。并成功在大肠杆菌细胞中可溶性表达,在ELISA检测中显示与D-二聚体结合的优势。结论:该抗体株D13可供进一步开展导向溶栓剂的研究。Objective： Selecting Fab phage antibodies to D-dimer from the phage antibody library,to construct a kind of humanized target-thrombolytic antibody. Methods： A human phage-displayed Fab library was constructed by using phage surface display techndogy. Through biopanning, employing D-dimer as antigen, we screened antibodyphages which interacted with D-dimer. Then the obtained antibodies were expressed in XL1-Blu. An ELISA test was done to assure the D-dimer-binding activity of the antibodies. Results： ELISA verified that the positive phage antibodies were specific against D-dimer. The positive phage antibody D13 was solutibility expressed in E.coli XL1-Bh. And The ELISA result showed that D13 had a prior binding activity to D-dimer. Conclusions： The success of isolating human anti-Ddimer Fab D13 provides an alternative base to make oriented thrombolytic.

关 键 词：人源抗D-二聚体抗体 噬菌体展示 导向溶栓 

分 类 号：R392[医药卫生—免疫学]