中国人群HIV-1B亚型Nef蛋白特异性细胞毒性T细胞反应的研究  

Analysis of HTV-1 clade B Nef specific CD8^+ T cell responses in Chinese population

作　　者：庄严[1] 翟嵩[1] 王少扬[1] 李新红[1] 康文臻[1] 于旭[2] Marcus Altfeld Bruce Walker 孙永涛[1] 

机构地区：[1]第四军医大学唐都医院全军感染病诊疗中心,西安710038 [2]美国哈佛大学医学院麻省总院艾滋病研究中心

出　　处：《中华微生物学和免疫学杂志》2006年第6期549-552,共4页Chinese Journal of Microbiology and Immunology

基　　金：美国NIH资助(NIH Contract No.NOL-AL-30024)

摘　　要：目的探讨中国人群HIV-1B亚型Nef蛋白特异性细胞毒性T细胞(CTL)反应特征及其与病毒载量以及CD4细胞数量间的关系。方法选取33例HIV-1B亚型感染者。用合成的HIV-1B亚型Nef全基因序列肽库作为抗原,ELISPOT方法检测HIV-1B亚型Nef蛋白特异性CTL反应,同时测定病毒载量及CD4细胞数量。结果70％的感染者对Nef产生特异性CTL反应,单一肽段能够被识别的频率不超过40％,应答强度为(1102±2136)SFC(斑点形成细胞数)／106 PBMC。HIV-1B亚型Nef特异性CTL应答的强度和频率之间没有显著的相关性。HIV-1 Neff特异性CTL反应强度与病毒载量间存在显著负相关,与CD4细胞数量间存在显著正相关。结论初步确定了Nef蛋白CTL应答的优势区域。这些区域主要集中在一些高度保守的氨基酸序列。提示HIV-1B亚型Nef特异性CTL应答在疾病进展中对机体具有保护性作用。Objective To analyse the character of HIV-1 clade B Nef specific CD8^＋ T cell responses in Chinese population and the relationship among the responses, viral load and CD4 cell counting. Methods HIV- 1B Nef specific CD8^＋ T cell responses were analyzed by gamma interferon （IFN-γ） enzyme-linked immunospot （ELISPOT） assay using 27 synthetic peptides spanning all HIV-1B Nef protein. Viral load and CD4 cell counting were also tested. Results At least 1 peptide was recognized by 70% subjects. The magnitude of responses was 1102 ＋ 2136 SFC/106 PBMC. Magnitude of the HIV-1B Nef specific CD8^＋ T cell responses was positively correlated with plasma viral load and negatively correlated with CD4 cell counting. Conclusion Several immunodominant regions were identified for screening CD8^＋ T cell epitopes. These regions were mainly clustered in highly conserved regions of Nef genes. HIV-1B Nef specific CD8^＋ T cell responses could prevent disease progression in HIV infection.

关 键 词：人免疫缺陷病毒 NEF 细胞毒性T淋巴细胞 

分 类 号：R392[医药卫生—免疫学]