小尾寒羊高繁殖力候选基因RBP4的研究  

Study on RBP4 as a Candidate Gene for Prolificacy of Small Tailed Han Sheep

作　　者：何远清[1] 郭晓红[2] 王金玉[1] 储明星[3] 方丽[3] 叶素成[3] 周忠孝[4] 

机构地区：[1]扬州大学动物科学与技术学院,扬州225009 [2]河南科技大学动物科技学院,洛阳471003 [3]中国农业科学院畜牧研究所,北京100094 [4]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2006年第7期646-649,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："863"国家高技术研究发展计划(2002AA211081)

摘　　要：以视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因为候选基因,采用PCR-SSCP技术分析了RBP4基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(多赛特羊、萨福克羊)中的单核苷酸多态性,同时研究这个基因对小尾寒羊高繁殖力的影响。结果表明:RBP4基因扩增片段在4个绵羊品种中存在PCR-SSCP多态性。BB基因型只出现在高繁殖力绵羊品种中,而低繁殖力绵羊品种则没有BB基因型;AB基因型频率随着绵羊繁殖力的降低而升高;AA基因型只出现在小尾寒羊、多赛特羊中。BB基因型小尾寒羊产羔数分别比AA和AB基因型多0.52只(P<0.05)和0.67只(P<0.05),AA和AB基因型小尾寒羊产羔数没有显著差异(P>0.05)。本研究检测的绵羊RBP4基因座位与小尾寒羊高繁殖力相关。Retinol binding protein 4（RBP4）gene was studied as a candidate gene on the prolificacy of Small Tailed Han sheep. The single nucleotide polymorphism of RBP4 gene in both high fecundity sheep breeds （Small Tailed Han sheep, Hu sheep） and low fecundity sheep breeds （Dorset sheep, Suffolk sheep） was analyzed by PCR-SSCP. There was PCR-SSCP polymorphism in four sheep breeds. BB genotype was only detected in high fecundity sheep breeds, AA genotype was only detected in Small Tailed Han sheep and Dorset sheep. With decreasing fecundity in sheep, AB genotype frequency increased. The ewes with genotype BB had 0.52 （P〈0.05） and 0. 67 （P〈0.05） lambs more than those with genotype AA and AB in Small Tailed Han sheep, respectively. These results showed that the studied loci of RBP4 gene had relationship with high fecundity of Small Tailed Han sheep.

关 键 词：小尾寒羊 高繁殖力 视黄醇结合蛋白4基因 PCR-SSCP 

分 类 号：S826.2[农业科学—畜牧学]