不同品种猪肌肉组织心型脂肪酸结合蛋白基因的表达差异  

Porcine H-FABP Gene Expression in Different Genotypes and Muscular Tissues

作　　者：罗献梅[1] 陈代文[1] 张克英[1] 

机构地区：[1]四川农业大学动物营养研究所,雅安625014

出　　处：《畜牧兽医学报》2006年第7期727-730,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"973"项目(2004CB117500)

摘　　要：用体重约100 kg DLY杂交猪和雅南猪各5头,屠宰取骨骼肌和心肌样品,采用RT-PCR方法研究心型脂肪酸结合蛋白(H-FABP)基因在不同品种猪肌肉组织表达的差异。结果表明:H-FABP基因表达于猪的骨骼肌和心肌中,但是其在2种肌肉组织中的表达量不同,心肌中表达量极显著高于骨骼肌(P<0.01);心肌和骨骼肌H-FABP基因的表达量2品种猪差异不显著(P>0.05),但无论是心肌还是骨骼肌雅南猪的表达量都高于DLY杂交猪。Porcine H-FABP gene expression diversity was investigated in cardiac and skeletal muscles of DLY（Duroc × Landrance × Yorkshire） and Ya＇ nan pigs weighed 100 kg by using RT-PCR. To collect the samples , five pigs were slaughtered in each genotype. The results indicated that HFABP gene expressed both in porcine cardiac and skeletal muscles. The expression in cardiac muscle was more active than in skeletal muscle （P〈0.01）. However, it was identified that HFABP gene expression in Ya＇nan pig was a little higher than in DLY both in cardiac and skeletal muscles （P〉0.05）.

关 键 词：RT-PCR H-FABP 基因表达 猪 肌肉 

分 类 号：S826.2[农业科学—畜牧学]