LMP2基因多态性及夫妻共享率与重度子痫前期的相关性研究  

Determine the polymorphisms and parent′s gene-sharing rate of LMP2 in women with pre-eclampsia and the relationship with severe pre-eclampsia

作　　者：吴爱华[1] 高眉扬[1] 曾林涓[2] 陈盛强[3] 佘达贤[1] 

机构地区：[1]广州医学院第二附属医院产科,510260 [2]广东省珠海市中山大学第五附属医院血液科 [3]广州医学院第二附属医院中心实验室

出　　处：《中华围产医学杂志》2006年第3期154-156,共3页Chinese Journal of Perinatal Medicine

基　　金：广东省卫生厅资助项目(A2001278);广东省自然科学基金(04009570);广州市教育局资助项目(01-12)

摘　　要：目的 测定重度子痫前期（severe pre-eclampsia，S-PE）患者和正常孕妇多功能蛋白酶（large muhifunctional protease，LMP2）基因多态性及夫妻共享率，探讨该基因与S-PE发病的相关性。方法 选择102例S-PE患者及其配偶为研究对象，随机选择200例正常孕妇及其配偶作为对照。所选孕妇均为单胎初孕，孕35～40周。各取2ml外周静脉血抽提DNA，应用PCR—RFLP方法进行LMP2基因分型。结果 LMP2R／H在S-PE患者中的分布频率显著高于正常孕妇组（53．9％和35．5％，P〈0．01），LMP2R／R在S-PE患者中的分布频率显著低于正常孕妇组（41．2％和58．5％，P〈0．01）。LMP2R／H在S-PE组的夫妻共享率与正常孕妇组的相比显著增高（24．5％和10．5％，P〈0．01）。结论 LMP2R／H很可能为S-PE发病的易感基因；而LMP2R／R可能是S-PE的保护基因。Objective To study the polymorphisms of the large mutiplefunctional protease （LMP2） genes in women with severe pre-eclampsia （S-PE） and the sharing rate of LMP 2 in these women and their partners and couples. Methods Totally, 102 S-PE women and their husbands were enrolled in the study group, and 200 normal pregnant women and their husbands as control. The types of LMP2 alleles were assessed by polymerase chain reaction-restriction fragment lengthpolymorphism method （PCR-RFLP） after PCR. Results The frequency of LMP2R/H in S-PE group was significantly higher than that of the control （53.9% vs 35. %, P〈0.01）, but the frequenay of LMP2 R/R was lower （41.2% vs 58.5%, P〈0.01）. The LMP2R/H gene compatibility between the partners in S-PE group was significantly increased than that of the controls （24.5 % vs 10.5, P〈0.01）. Conclusions LMP2R/H is a susceptible gene for pre-eclampsia, while LMP2R/R have protective effect against pre-eclampsia.

关 键 词：先兆子痫 多功能蛋白酶 多态性 限制性片段长度 

分 类 号：R714.246[医药卫生—妇产科学]