两种单克隆抗体荧光免疫偏振法测定人全血中环孢素浓度的比较  

Comparison of TDx and AxSYM monoclonal whole blood cyclosporine assay

作　　者：焦正[1] 张明[1] 仲珑瑾[1] 施孝金[1] 钟明康[1] 

机构地区：[1]复旦大学附属华山医院,上海200040

出　　处：《中国医院药学杂志》2006年第7期789-791,共3页Chinese Journal of Hospital Pharmacy

基　　金：上海市卫生局百人计划项目II期(编号:98BR003)

摘　　要：目的：考察TDx与AxSYM单克隆抗体荧光免疫偏振法测定全血中环孢素A浓度结果的差异。方法：采集12名肾移植患者服药后12h内不同时间点的血样。样本分别用TDx与AxSYM进行测定。测定结果通过均数比较、Passing—Bablok回归法和Bland-Altman偏差图法进行分析。结果：TDx和AxSYM两法的测定结果相关。服药后0．5～4h的血样，两法测定结果差异无显著性。而服药6h后两法的测定结果差异有显著性，且TDx的测定结果高于AxSYM。结论：TDx与AxSYM法测定人全血中环孢素浓度的结果有差异，且与采样时间有关。OBJECTIVE To compare the difference between TDx and AxSYM monoclonal fluorescence polarization immunoassay in determination of cyclosporine A （CsA） in human whole blood. METHODS Blood samples were collected before and after administration of CsA in a period of 12 hours from 12 renal transplant patients. CsA concentrations were determined by TDx and AxSYM assay. Mean comparison, Passing-Bablok regression method and Bland-Airman plots were employed to investigate the difference of the two assay methods. RESULTS There is a good relation between two assays. The results of TDx and AxSYM have no significant difference during 0. 5 - 4 h postdose, while the results observed by TDx were higher than AxSYM in terms of 6- 12 h postdose. CONCLUSION The difference between TDx and AxSYM assay methods, is due to the sampling time.

关 键 词：环孢素A 荧光免疫偏振法 治疗药物监测 

分 类 号：R917[医药卫生—药物分析学]