新鱼腥草素钠滴眼液对家兔细菌性角膜炎的影响  

Effect of new sodium houttuyfonate eye drops on experimental rabbit bacterial keratitis

作　　者：陈效[1] 徐永红[2] 王章元[1] 孙汉清[1] 

机构地区：[1]华中科技大学同济医学院药学院,湖北武汉430030 [2]武汉大学人民医院眼科,湖北武汉430060

出　　处：《中国医院药学杂志》2006年第7期820-823,共4页Chinese Journal of Hospital Pharmacy

摘　　要：目的:研究新鱼腥草素钠滴眼液对家兔细菌性角膜炎的治疗作用。方法:于家兔角膜实质层分别注射接种金黄色葡萄球菌和绿脓杆菌制成两种角膜炎模型。接种后连续6d分别以不同剂量新鱼腥草素钠滴眼液进行治疗。同期肉眼观察记录眼部症状,于第6天取角膜分别作病理切片和细菌培养。结果:新鱼腥草素钠滴眼液各剂量组中,金黄色葡萄球菌和绿脓杆菌所致角膜炎的积脓、红肿、角膜浑浊、溃疡等肉眼症状以及角膜增厚、溃疡、炎细胞浸润、成纤维细胞增生等镜下炎症表现均得到明显改善;24h角膜细菌培养菌落计数也均显著低于模型组。病理切片所示以及细菌培养菌落计数均与肉眼症状评分相一致。结论:新鱼腥草素钠滴眼液可抑制病原菌在眼表局部增殖,并能促进角膜修复愈合,能有效控制细菌感染所致的角膜炎症。表明本药具有临床应用前景。OBJECTIVE To study the effect of new sodium houttuyfonate eye drop on rabbit bacterial keratitis models caused by staphylococcus aureus and bacillus pyocyaneus. METHODS Rabbit keratitis models were established by intrastroma injection of culture medium containing either bacillus pyocyaneus or staphylococcus aureus, new Sodium Houttuyfonate eye drops were topically applied to corneas 6 hours following bacterial injection, 6 days on end. Macroscopical symptoms of keratitis were observed and graded everyday throughout the course of the experiment. Corneas were taken out on the 6^th day for either pathological staining or bacterial culture. RESULTS Both staphylococcal and bacillary, either macroscopically or microscopically the keratitis symptoms significantly abated in SNHED treated groups compared with models. And after 24 hours of culturing SNHED treated corneas gave rise to markedly less colony forming units than model corneas. CONCLUSION new Sodium houttuyfonate eye drops are effective in alleviation of experimental keratitis caused by staphylococcus aureus and bacillus pyocyaneus. And the drug is capable of containing local bacterial proliferation and promoting corneal healing.

关 键 词：新鱼腥草素钠 角膜炎 金黄色葡萄球菌 绿脓杆菌 

分 类 号：R965[医药卫生—药理学]