羧甲基壳多糖对瘢痕疙瘩成纤维细胞增殖和胶原合成的影响  

Effect of carboxymethyl-chitosan on proliferation and collagen synthesis in keloid fibroblasts in vitro

作　　者：陈海蓉[1] 李春霞[1] 陈西广[2] 吴延芳[3] 

机构地区：[1]青岛市市立医院皮肤科,青岛266011 [2]中国海洋大学生命学院 [3]青岛大学医学院附属医院

出　　处：《中华医学美学美容杂志》2006年第3期165-168,共4页Chinese Journal of Medical Aesthetics and Cosmetology

摘　　要：目的观察羧甲基壳多糖(carboxymethyl-chitosan,CM-CH)对体外培养的瘢痕疙瘩成纤维细胞(keloid fibroblasts,KFB)增殖和胶原合成的作用,探讨其治疗瘢痕疙瘩的机制。方法采用四噻唑蓝(methylthiazoletrazolium,MTT)测定法和羟脯氨酸比色法(hydroxyproline,HP)测定不同浓度CM-CH作用KFB后对其增殖和上清液中胶原合成的影响。结果CM-CH在10、50、100、200μg/ml作用KFB48、72h后,对KFB增殖有明显抑制作用(P<0.05)。200μg/ml作用24、48、72h后,对KFB增殖抑制作用与曲安萘德组比较差异无统计学意义(P>0.05)。CM-CH在10、50、100、200μg/ml对KFB作用48h后,能显著抑制该细胞胶原的合成(P<0.01)。100、200μg/ml CM-CH抑制作用与曲安萘德组比较差异无统计学意义(P>0.05)。结论CM-CH对体外培养的KFB增殖和胶原合成有明显抑制作用,从而提示该物质治疗瘢痕疙瘩具有潜在前景。Objective To investigate the effects of carboxymethyl-chitosan （CM-CH） on keloid fibroblasts （KFB） proliferation and collagen synthesis. Methods Keloid fibroblasts were isolated from fresh keloid tissue and cultured. The effect of CM-CH on proliferation was examined by MTT assay. The synthesis of collagen was evaluated by hydroxy proline （HP） colorimetric analysis. Results The fibroblasts were treated with CM-CH at concentration of 10 μg/ml, 50 μg/ml, and 100 μg/ml 48h and 72h after treatment, and all of the three concentrations showed inhibitory effects significantly （P〈0.05）. At concentration 200 μg/ml the significant inhibitory effect on KFB proliferation was observed at any detected time point （P〈0. 05）. The inhibitory effect of CM-CH on KFB was similar to that of triamcinolone acetonid （P〉0.05） at 200 μg/ml concentration after 24h, 48h, and 72h. CM-CH at concentration of 10 μg/ ml, 50 μg/ml, and 100 μg/ml after treatment for 48h on KFB could markedly decrease the synthesis of collagen （P〈0.01）. Conclusions The above results demonstrate that CM-CH could significantly inhibit the proliferation and collagen synthesis in cultured KFB. It may be developed as a new drug for the treat- ment of keloids.

关 键 词：羧甲基壳多糖 瘢痕疙瘩成纤维细胞 增殖 胶原合成 

分 类 号：R758.6[医药卫生—皮肤病学与性病学]