碱性成纤维细胞生长因子对牵张性脊髓损伤后ChAT表达的影响  

Effect of basic fibroblast growth factor on ChAT expression in tractive spinal cord injury in rats

作　　者：刘雷[1] 唐康来[1] 杨柳[1] 李起鸿[1] 

机构地区：[1]第三军医大学西南医院关节外科,重庆400038

出　　处：《第三军医大学学报》2006年第14期1493-1494,共2页Journal of Third Military Medical University

摘　　要：目的观察碱性成纤维细胞生长因子(bovine basic fibrob last growth factor,bFGF)对牵张性脊髓损伤后神经元内ChAT表达的影响。方法建立大鼠牵张性脊髓损伤动物模型,治疗组分别于术后即刻、1、2、3、4、8、12、24 h经细导管注入bFGF溶液20μl(含bFGF 20μg),对照组在相同时间注入等量生理盐水,然后于术后4、7、14 d处死取材。分别于术前及术后4、7、14 d行胆碱乙酰转移酶(ChAT)的免疫组化检测。结果牵张性脊髓损伤后ChAT阳性细胞数持续下降,经bFGF处理的大鼠ChAT阳性细胞数增多,治疗组与对照组比较差异有显著性(P<0.05)。结论bFGF能促进脊髓神经元合成ChAT,从而促进动物运动功能的恢复。Objective To observe the effect of basic fibroblast growth factor on ChAT expression in neurons after tractive spinal cord injury in rats. Methods The animal model of tractive spinal cord injury in 36 SD rats was established. The bFGF-treated rats （ n = 18 ） received 20 μl bFGF solution containing 20 μg bFGF into subarachrioid cavity immediately, or at 1,2, 3, 4, 8, 12 and 24 h after animal model establishment, while the saline-treated rats （ n = 18 ） received the equal volume of normal saline at the same time. Another 6 normal SD rats underwent no treatment as normal controls. All rats were killed on postoperative day 4, 7, 14. The choline acetyltransferase （CHAT） were detected by immunohistochemistry preoperatively and on day 4, 7, 14 after spinal cord injury. Results The number of neurons positive of ChAT decreased gradually after tractive spinal cord injury. After bFGF treatment, positive neurons of ChAT increased in number, with significant difference between the bFGF-treated group and saline-treated group （ P 〈 0. 05 ）. Conclusion bFGF can enhance the synthesis of motor nerve transmitter, acetylcholine, and facilitate the motor function recovery of animal models.

关 键 词：牵张 脊髓损伤 胆碱乙酰转移酶 碱性成纤维细胞生长因子 

分 类 号：R338.1[医药卫生—人体生理学] R651.2[医药卫生—基础医学]