重庆地区汉族人群CD14基因启动子区-159T→C多态性及其与LPS反应分析  

Analysis of CD14-159 T→C polymorphism in Chongqing Han population and their relationship with LPS response

作　　者：董蕻[1] 蒋建新[1] 段朝霞[1] 顾玮[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所第四研究室,重庆400042

出　　处：《第三军医大学学报》2006年第15期1563-1565,共3页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划资助项目("973"项目)(G199905-4203);国家杰出青年科学基金资助项目(30325040)~~

摘　　要：目的观察重庆地区汉族人群CD14基因启动子区-159单核甘酸多态性(single nuc leotide polymorph ism,SNP)分布特征,及其与CD14表达和脂多糖(lipopolysaccharide,LPS)反应的关系。方法选择298例重庆地区汉族健康个体,采用限制性片段长度多态性法(restrictionfragm ent lengthpoly-morph ism,RFLP)进行基因分型,检测LPS刺激前后外周血单核-巨噬细胞mCD14荧光强度和血浆sCD14浓度。结果重庆地区汉族人群CD14-159 T/T、T/C、C/C基因型频率分别为32.21%、50.67%和17.11%,样本符合Hardy-W e inberg平衡,正常情况下,不同基因型mCD14水平差异不明显,而sCD14浓度有显著性差异(P<0.05),LPS刺激能增强mCD14和sCD14的表达,但各基因型的增强幅度没有明显差异。结论重庆地区汉族人群CD14-159 SNP等位碱基以T为主,T→C变异可抑制sCD14的表达,但对mCD14表达和LPS刺激反应的影响可能不明显。Objective To study the distribution of CD14 T （ - 159 ） C polymorphism in Chongqing Han population, and their relationship with the expression of CD14 and LPS response. Methods PCR-RFLP were used for genotyping in 298 volunteers from Chongqing Han population, and the levels of mCD14 and sCD14 in peripheral blood were detected before and after LPS stimulation. Results Frequency of T/T, T/C and C/C was 37.93%, 45.81% and 16.26% , respectively. Sample used met Hardy-Weinberg equilibrium. There were no difference in mCD14 between genotypes with or without LPS, but there were marked difference in sCD14. Conclusion T is the predominant allele in CD14 - 159 SNP in Chongqing Han population. - 159 T→C mutation could inhibit the expression of sCD14, but may not play an important role in the expression of mCD14 and LPS response.

关 键 词：CDL4 SNP RFLP Hardy-Weinberg平衡 LPS 

分 类 号：R181.34[医药卫生—流行病学] R394.5[医药卫生—公共卫生与预防医学]