局灶性皮质发育障碍模型鼠大脑皮质病灶组织的超微结构观察  

Ultrastructural changes of focal tissue in cerebral cortex of disorders of cortical development in rats

作　　者：马勋泰[1] 晏勇[1] 王学峰[1] 罗春阳[2] 王晓平[3] 晏宁[4] 

机构地区：[1]重庆医科大学附属第一医院神经内科重庆市神经病学重点实验室,重庆400016 [2]重庆市中山医院神经内科,重庆400013 [3]四川省人民医院神经内科,成都610031 [4]重庆市第三人民医院神经内科,重庆400014

出　　处：《第三军医大学学报》2006年第15期1570-1572,共3页Journal of Third Military Medical University

基　　金：重庆市教委应用基础研究基金资助项目[渝教科(2001)12-29];重庆市医学科技基金资助项目(01-1-013)~~

摘　　要：目的探讨皮质发育障碍(d isorders of cortical developm ent,DCD s)模型鼠小脑回(又名小的脑回或微脑回)周围病灶组织的电镜、病理特征,分析其与癫痫发生的关系。方法9只健康新生W istar雄性大鼠分为正常对照组、液氮损伤组和假手术组,每组3只,建立液氮损伤诱导的局灶性DCD s模型大鼠,观察其行为学和脑电图的变化,行HE、N issl染色,在解剖镜下取材,透射电镜下观察并照像。结果正常对照组和假手术组大鼠无任何抽搐发作,液氮损伤组偶有抽搐发作,但大多表现为活动增多、兴奋躁动、搔抓和“洗脸样活动”频繁。皮层电极EEG示小波幅节律为主,无典型的尖波、棘波、尖慢波、棘慢综合波发放。电镜所见DCD s鼠微脑回周围锥体神经元内线粒体肿胀,内质网扩张,胶质细胞肿胀,毛细血管周围水分积聚,部分髓鞘退变,异常神经纤维网,突触未见明显异常。结论小脑回周围病灶组织的超微结构病变可为局灶性DCD s所致癫痫提供病理基础。Objective To study the pathological characteristics of disorders of cortical development （DCDs） and to analyze the relationship between the characteristics and epileptogenesis. Methods A total of 9 newborn healthy male Wistar rats were randomly divided into three groups including freeze-lesioned, sham-operated and normal groups. The disorders of cortical development model was established by liquid nitrogen lesion. All rats in the three groups were raised for 16 to 18 weeks after operation, then the focal cerebral tissues were taken and observed by transmission electron microscope （TEM） as well as histochemistry （HE and Nissl stain）. Results Seizures did not occur in normal rats or sham-operated rats, while in freeze-lesioned rats besides seizures by chance, the main display was more activities, exciting, restlessness, and frequent action of face-wash. EEG of freeze-lesioned rats showed no typical sharp waves, or spikes. All freeze-lesioned rats displayed typical cortical malformation a longitudinal microgyrus. The electron microscopic examination showed mitochondrial lesions, abnormal neuropil and endoplasmic reticulum expand in neurons and myelinoclasis in neurogliocytes. The changes were more severe around microgyria. Conclusion DCDs lead to main lesion around microgyrus. These changes could be one of mechanisms in an intrinsic epileptogenicity of cortical dysplasias from the pathological characteristics by electron microscopy.

关 键 词：皮质发育障碍 局灶性 病理学 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R329.27[医药卫生—基础医学]