利用噬菌体有效分离稀有放线菌的初步研究  

Preliminary study on an effective method for isolation of rare actinomycetes using phages

作　　者：段淑蓉[1] 苏芸芸[1] 张玉琴[1] 陈义光[1] 徐丽华[1] 

机构地区：[1]云南大学云南省微生物研究所教育部微生物资源重点实验室

出　　处：《云南大学学报（自然科学版）》2006年第4期354-357,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(30270004)

摘　　要：利用噬菌体S7和S3专一性感染裂解链霉菌的特性,选用几丁质、土壤浸汁和改良海藻糖-天门冬酰胺3种分离培养基,结合使用预培养、预处理及添加不同抑制剂等多种手段,对采自云南、缅甸的3份土样进行处理.实验检测了在不同培养基上噬菌体对链霉菌的抑制效果,结果表明在几丁质培养基上,噬菌体对链霉菌的抑制效果明显,提高了稀有放线菌的出菌率,增加了物种多样性.利用噬菌体是一种有效分离稀有放线菌的方法.Both S3-phage and S7-phage specially infect Streptomyces. Chitin agar, soil exact agar and improved trehalose-asparagine agar were choosed as isolation media. Three soil samples respectively from Yunnan, China and Myanmar are treated using preincubation, pretreatments and inhibitors. That the phages inhibit growth of Streptomyces on different media specially on chitin agar is proved by this study. The rate of rare actinomyces increased and the variety improved. It is a useful method to utilize phages in selective isolation of

关 键 词：稀有放线菌 分离方法 噬菌体 

分 类 号：Q93[生物学—微生物学]