农杆菌介导玉米转基因的影响因素研究  

Influencing factors on maize transgene process mediated by Agrobacterium

作　　者：白云凤[1] 王国英[1] 苟明月[1] 赵晋锋[1] 董志刚[1] 

机构地区：[1]中国农业大学农业生物技术国家实验室

出　　处：《中国生态农业学报》2006年第3期173-175,共3页Chinese Journal of Eco-Agriculture

基　　金：国家高技术发展(863)计划项目(2001AA212051)资助

摘　　要：研究了农杆菌介导玉米转基因过程中涉及的外植体选择、高渗和农杆菌共培养、选择培养基筛选转化体等因素对愈伤组织生长及转化效率的影响。结果表明,同一基因型玉米自交系中有少数幼穗幼胚诱导出的愈伤组织生长速度快,转化效率高;高渗和农杆菌共培养以及除草剂筛选抑制愈伤组织的生长,致使部分转基因愈伤不能分化成苗,并提出其解决途径。The effects of explant selection, high osmotic treatment, co-culture with Agrobacterium, screening of transformed plants by selecting medium on the callus growth and transformation efficiency were studied in the process of maize transformation mediated by Agrobacterium. The results show that the growth rate and transformation efficiency of immature embryos derived from a vigorous ear are higher than those from other ears. High osmotic treatment and co-culture with Agrobacteriurn and herbicide screening impede the callus growth. The solutions are put forward.

关 键 词：玉米转基因 农杆菌介导 除草剂 转化效率 

分 类 号：S511[农业科学—作物学] S513