金氏痔疮膏对人脐静脉内皮细胞的抑制作用  

Inhibitory Effect of Jin’s Hemorrhoid Ointment on Human Umbilical Vein Endothelial Cell

作　　者：鲍银童[1] 刘长宝[1] 金纯[1] 金可可[2] 金定国[1] 

机构地区：[1]温州医学院附属第二医院中西医结合肛肠科,浙江温州325027 [2]温州医学院,浙江温州325035

出　　处：《中国中医药信息杂志》2006年第7期15-16,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家中医药管理局科学技术研究专项(02-03ZL06)

摘　　要：目的观察金氏痔疮膏精提液对人脐静脉内皮细胞(ECV-304)增殖的影响,探讨其可能的治痔作用机制。方法采用MTT法检测不同浓度的金氏痔疮膏精提液和组方中单味中药对ECV-304细胞增殖的影响。结果不同浓度的金氏痔疮膏精提液对ECV-304细胞增殖均有抑制作用,且呈剂量依赖性;高、中、低浓度的大黄煎液和高浓度的姜黄对ECV-304细胞增殖有抑制作用。结论金氏痔疮膏具有抑制人脐静脉内皮细胞增殖的作用;大黄是金氏痔疮膏中发挥抑制血管内皮细胞作用的主药,姜黄亦起了一定作用。Objective To investigate the inhibition of Jin＇s hemorrhoid ointment extract on proliflation of human umbilical vein endothelial cell （ECV-304）, then to study the mechanism of treating hemorroid. Methods MTT assay was used to observe the inhibition of various concentrations Jin＇s hemorrhoid ointment extract and single Chinese Herbs of compound on human umbilical vein endothelial cell （ECV-304）. Results Various concentrations Jin＇s hemorrhoid ointment extracts inhibited proliflation of human umbilical vein endothelial cell, which depended on concentration. All of Rheum decoctions and high concetration Curcuma decoction inhibited ECV-304 proliflation. Conclusion Jin＇s hemorrhoid ointment can inhibit proliflation of human umbilical vein endothelial cell, and Rheum maybe the main effective part of Jin＇s hemorrhoid ointment, Curcuma also take part in it.

关 键 词：金氏痔疮膏 大黄 姜黄 人脐静脉内皮细胞 MTT法 

分 类 号：R289.11[医药卫生—方剂学]