RP-HPLC法测定消炎退热贴中栀子苷的含量  

RP-HPLC Determination of Geniposide in Xiaoyan Tuire Patch

作　　者：韩建伟[1] 乔明[1] 付敏[1] 刘超英[1] 冯德有[1] 

机构地区：[1]湖北中医学院药学院,湖北武汉430061

出　　处：《中国中医药信息杂志》2006年第7期50-51,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的建立高效液相色谱法测定消炎退热贴中栀子苷含量的方法。方法采用AgilentZORBAXSB-C18(4.6mm×250mm,5μm)色谱柱;以水-乙腈-磷酸(86∶14∶0.01)为流动相;流速1mL/min;检测波长238nm;柱温30℃。结果栀子苷的线性范围0.07～0.70μg(r=0.99997),平均回收率为98.60%,RSD=1.96%。结论该方法简便灵敏,结果准确,可用于消炎退热贴制剂的含量测定。Objective To establish a RP-HPLC method to determine the content of geniposide in Xiaoyan Tuire Patch. Method The column of Agilent ZORBAX SB-C18 （4.6 mm×250 mm 5×m） was used. The mobile phase was composed of water-acetonitrile-phosphoric acid （86 ： 14： 0.01）. The flow rate was 1 mL/min, with UV detection at 238 nm, and the temperature was 30℃. Results The linear range was 0.07-0.70μg （r=0.99997）. The average recovery was 98.60% and the RSD was 1.96%. Conclusion The method is simple, sensitive and accurate, and can be used for the determination of Xiaoyan Tuire Patch.

关 键 词：消炎退热贴 栀子苷 反相高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]