瘦素基因启动子G-2548A多态性与冠心病的关系  

The study of leptin gene polymorphisms G-2548A in patients with coronary heart disease

作　　者：赵勇[1] 王洪杰[1] 曾和松[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院心内科,湖北武汉430030

出　　处：《临床内科杂志》2006年第7期489-491,共3页Journal of Clinical Internal Medicine

基　　金：国家自然科学基金资助项目(30470713)

摘　　要：目的探讨中国湖北地区汉族人群瘦素基因启动子2548编码处碱基G／A多态性与冠心病（CHD）的关系。方法（1）选择CHD患者156例（CHD组），其中急性冠脉综合症80例，稳定性CHD76例，同时入选心电图检查无异常或冠脉造影正常者150例（对照组）。（2）应用聚合酶链反应及限制性片段长度多态性分析技术，分析CHD患者和对照组的瘦素基因型。结果CHD组与对照组中瘦素基因启动子G-2548A编码处A等位基因比较差异有非常显著性（X^2=11．55，P〈0．01）。基因型频率的相对危险度分析发现，AA加AG基因型患CHD的危险度是GG基因型的3．827倍（OR=3．827，CI=1．672—8．756）。结论瘦素基因启动子G-2548A的多态性与CHD易感性有关，A等位基因可能是CHD发病的遗传易感因素。Objective To explore whether single nucleotide polymorphism G/A in the promoter of leptin gene related to CHD in Chinese Han peoples of Hubei province. Methods The genotypes of leptin were detected by polymerase chain reaction-Restriction fragment length polymorphism （PCR-RFLP） methods in 156 CHD including 80 ACS patients and 76 SCHD patients,and 150 controls. Results There was significance difference in frequencies of allele in G-2549A polymorphism between CHD and control group （CHD：control X^2 = 11.55 ,P 〈 0.01 ）o The relative risk suffering from CHD with genotype AA ＋ AG was 3. 827 times of those with genotype GG（ OR = 3. 827, CI = 1. 672 - 8. 756）. Conclusion Leptin gene polymorphism G-2548A is significantly correlation with the CHD. A allele of leptin may be a genetic factor that may contribute to individual susceptibility for CHD.

关 键 词：冠状动脉疾病 瘦素 基因型 限制性片段长度多态性 

分 类 号：R541.4[医药卫生—心血管疾病]