应用巢式PCR-RFLP方法鉴别微小隐孢子虫、安氏隐孢子虫和火鸡隐孢子虫的研究  

he differentiation of Cryptosporidium parvum,C.andersoni,and C.meleagrides by Nested PCR-RFLP analysis of the SSU rRNA gene

作　　者：姚龙泉[1,2] 张西臣[1] Lihua Xiao 李建华[1] 尹继刚[1] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]沈阳农业大学畜牧兽医学院,辽宁沈阳110161 [3]Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia 30341, USA

出　　处：《中国预防兽医学报》2006年第4期446-449,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金资助项目[国科金生外(2002)30210403203]

摘　　要：应用巢式聚合酶链反应(NestedPCR)-限制片段长度多态性(restrictionfragmentlengthpolymor-phism,RFLP)方法对微小隐孢子虫(C.parvum)、安氏隐孢子虫(C.andersoni)和火鸡隐孢子虫(C.melea-grides)的鉴别进行了研究。结果显示C.parvumBOCC2、C.andesoniBOCC2和C.meleagridesCHCC1扩增产物片段大小分别为830bp、828bp和828bp,扩增产物分别经VspⅠ酶切后形成3种不同的RFLP图谱,根据RFLP图谱可鉴别C.parvum、C.andersoni和C.meleagrides。本研究为我国隐孢子虫的分类和隐孢子虫病的分子流行病学研究打下了良好基础。Differentiation of Cryptosporidium parvum, C. andersoni and C, meleagrides was studied by Nested PCR-RFLP analysis of the SSU rRNA gene. The fragments of 830 bp, 828 bp and 828 bp were amplified by Nested PCR from the C. parvum BOCC2, C.andesoni BOCC2 and C. meleagfides CHCC1 respectively .Three different kinds of RFLP restriction maps were formed after PCR products were digested by Vsp I respectively. C. parvum , C. andersoni and C. meleagrides can be differentiated according to RFLP restriction maps. The study undertaked in the present experiment was suitable for the taxonomy of Cryptosporidium and molecular epidemiology survey of Cryptosporidiosis.

关 键 词：微小隐孢子虫 安氏隐孢子虫 火鸡隐孢子虫 巢式PCR-RFLP 

分 类 号：S852.654[农业科学—基础兽医学]