心肺复苏后犬脑组织S100B蛋白mRNA表达的变化及纳洛酮的干预  

THE VARIATION OF THE EXPRESSION OF S100B mRNA IN DOG’S CEREBRAL TISSUE AFTER CARDIOPULMONARY RESUSCITATION AND THE INTERVENTION OF NALOXONE

作　　者：许松青[1] 田朝伟[1] 江慧琳[1] 张弋[1] 林珮仪[1] 陈晓辉[1] 陈敏生[2] 

机构地区：[1]广州医学院第二附属医院,广东广州510260 [2]广州医学院,广东广州510260

出　　处：《现代医院》2006年第8期31-33,共3页Modern Hospitals

基　　金：广东省卫生厅资助项目;编号:A2005066

摘　　要：目的通过检测心跳骤停犬复苏后及纳洛酮干预后脑组织中S100B蛋白mRNA的表达情况,了解纳洛酮对脑复苏的影响。方法18只健康杂种犬,随机分成三组,每组6只,予体外电击诱发室颤,对照组:心跳骤停后予标准心肺复苏术;纳洛酮组:心跳骤停后予标准心肺复苏术加纳洛酮;空白组:不诱发室颤,于复苏后6h取脑海马组织行脑形态学检查及S100B蛋白mRNA表达的测定。结果纳洛酮组S100B蛋白mRNA表达明显低于对照组(p<0.01),纳洛酮组脑组织的病理损害低于对照组。结论使用纳洛酮后心肺复苏犬脑组织的病理损害有所减轻,脑组织S100B蛋白的表达显著减少,纳洛酮可能通过减少S100B蛋白的表达而减轻心肺复苏后脑的再灌注损伤。Objective To discuss the expression of S100B and the pathological change of dog＇ s cerebral tissue which were actualized by CPR, and the effect of naloxone on it. Methods 18 healthy adult cross dogs divided into three groups stochastically （ n = 6）. Blank group： Blank group were not induced ventricular fibrillation, collected the cerebral tissues after 6 hours; Control group： Control group were actualized standard CPR after cardiac arrest; Naloxone group ： Naloxone group were actualized standard CPR and used naloxone after cardiac arrest. The control group and naloxone group were collected the cerebral tissues after 6 hours when they have been actualized CPR and detect their S100B concentration and pathological change. Results The mRNA expression of cerebral S100B in control group were higher than those blank group （p 〈 0. 01 ） and naloxone group （p 〈 0. 01 ）. Control group presented obvious pathological damage of nerve cell and the damage of naloxone group was lower than the control group. Conclusion Naloxone can reduce the production of SIOOB in cerebral tissue and it＇ s pathological damage after cardiopulmonary resuscitation （ CPR）. and then reduce the nerve cell＇ s reperfusion injury.

关 键 词：心肺脑复苏 S100B蛋白 纳洛酮 犬 

分 类 号：R651.15[医药卫生—外科学] R541.78[医药卫生—临床医学]