小单孢菌FIM-203产生庆大霉素A的研究  

Gentamicin A as a Major Metabolite Produced by Micromonospora FIM-203

作　　者：郑卫[1] 魏天恩[1] 程振泰[1] 谢阳[1] 程元荣[1] 

机构地区：[1]福建省微生物研究所,福州350007

出　　处：《海峡药学》2006年第3期170-173,共4页Strait Pharmaceutical Journal

基　　金：福建省自然科学基金项目C 9810048

摘　　要：从福建武夷山森林土壤中分离到一株小单孢菌,编号为F IM-203,分类特征的初步研究表明该菌株属于棘孢小单孢菌。生物活性检测提示F IM-203的发酵液具有抗G+、G-细菌活性,发酵液经离子交换树脂提取获得的初提物含有3个组分,其中FW-203C为主要组分。理化性质和光谱学分析研究证明FW-203C与庆大霉素A(gen tam icin A)同质。A strain of Micromonosproa was isolated from a forest soil sample of Wuyi Mountain, Fujian and was designated as Micrornonospora FIM-203. The strain is classified as Micrornonospora echinospora FIM-203 exhibited activities against both gram-negative and gram-positive bacteria. A crude bioactive product was obtained by treating the broth with IRC-50（NH＋ form）resin and the major bioactive component FW-203C was purified from the crude product by GC-50（NH＋ form） cation ionic exchange resin chromatography. Physico-chemical and spectrometric studies proved that FW-203C is identical with Gentamicin A.

关 键 词：棘孢小单孢菌 氨基糖苷类抗生素 庆大霉素A 

分 类 号：R978.12[医药卫生—药品] R973.2[医药卫生—药学]