花生幼叶丛生芽的诱导和高效再生体系的建立  

Preliminary report on the in vitro culture of shoot induction from leaflets of peanut(Arachis hypogeae L.)

作　　者：李艳[1] 李少雄[1] 周桂元[1] 洪彦斌[1] 林坤耀[1] 梁炫强[1] 

机构地区：[1]广东省农科院作物研究所,广东广州510640

出　　处：《广东农业科学》2006年第7期14-16,共3页Guangdong Agricultural Sciences

基　　金：广东省农业攻关项目(2003B21203);广东省农业科学院重点攻关项目(04-攻关-02A)

摘　　要：以花生种子无菌条件下萌发4天、7天、10天、27天的幼叶作外植体,分别接种于加有不同激素配方的芽诱导培养基上,1周后叶片伤口处膨大并有黄绿色愈伤组织出现,3周左右可以看到丛生芽点的出现,芽诱导率达85%以上;继代培养4周后转至高浓度BA(10 mg/L)的MS培养基上促进芽伸长;当小苗长至3 cm左右时,移入NAA(1 mg/L)的MS培养基上诱导生根,获得完整的再生植株,再生率可以达到80%以上。The shoots were induced in vitro from peanut leaflets at 4 days, 7 days,10 days and 27 days old on MS medium with different plant growth regulator. The callus formed after 1 week, and the shoots were observed after 3 weeks, the highest rate of organgenesis was up to 85 %. The shoots grew further in MS medium with high BA concentration（ 10 mg/L） in about 4 weeks. At last the regeneration peanut seedling developed rooting on MS medium with NAA（ 1 mg/L）. The rate of regeneration was up to 80 %.

关 键 词：花生 离体培养 诱导 丛生芽 外植体 再生体系 

分 类 号：S685.99[农业科学—观赏园艺] S565.2[农业科学—园艺学]