蜂贝化瘀胶囊对糖尿病大鼠肾脏保护作用的实验研究  

EXPERIMENTAL STUDY ON PROTECTIVE EFFECT OF FENGBEIHUAYU CAPSULE ON KIDNEY OF RAT WITH DIABETES

作　　者：宫海民[1] 段文卓[2] 王术云[1] 金光香[2] 黄旭东[1] 

机构地区：[1]山东省潍坊市中医院,潍坊261041 [2]潍坊医学院,潍坊261042

出　　处：《中国中医药科技》2006年第4期220-221,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：山东省中医药管理局科研基金资助项目No.2001276

摘　　要：目的:探讨中药蜂贝化瘀胶囊对糖尿病(DM)肾脏的保护作用及其机制。方法:将Wistar大鼠分为DM组和中药治疗组,DM大鼠模型由链脲佐菌素(STZ)加高热量饮食诱导。治疗8周后,测定各组大鼠尿量、24小时尿蛋白、尿素氮、血肌酐、糖化血红蛋白、血清丙二醛、T细胞凋亡率和淋巴细胞FasL mRNA表达,并观察肾脏组织病理学改变。结果:DM组的尿量、24小时尿蛋白、尿素氮、血肌酐、糖化血红蛋白、血清丙二醛、FasL基因表达和T细胞凋亡率均明显高于正常对照组(P<0.05),形态学观察DM组大鼠肾脏有弥漫性肾小球硬化,肾小球系膜区增宽,肾小管基底膜增厚等病理改变;而中药治疗组的以上指标与DM组比较均明显减轻(P<0.05),并与正常对照组无显著性差异(P>0.05)。结论:蜂贝化瘀胶囊对DM大鼠肾脏病变有较好的防治作用,其机制可能与该方剂能抑制脂蛋白非酶基糖化、抑制脂质过氧化、控制功能基因的表达,减少T细胞凋亡等作用有关。Objective: To approach the protective effect and its mechanism of fengbeihuayu capsule on kidney of rat with diabetes.Methods:The model was induced by STZ and high caloric diet.Rats was divided into normal,control and fengbeihuayu capsule groups,the rat's urine volum,24h urinary protein,BUN,SCr,GHbAlc,MDA,apoptosis rate of T lymphocyte and its FasL mRNA expression were detected after treated 8 weeks.Results:The rat's urine volume,24h urinary protein,BUN,SCr,GHbAlc,MDA,apoptosis rate of T lymphocyte and its FasL mRNA expression were higher than those of normal group,the morphological observation showed that there was diffuse glomerular cirrhosis in kidney of diabetes group,meanwhile glomerular mesenteric region broaden,basement membrane of renal tubules thicken obviously,all these indexed were significantly improved in fengbeihuayu capsule group,there was no obvious difference between Chinese herb group and normal group.Conclusion:Fengbeihuayu capsule can prevent the renal lesions from diabetes mellitus.

关 键 词：糖尿病肾病/中医药疗法 @蜂贝化瘀胶囊/治疗应用 糖尿病肾病/病理学 肾/病理学 细胞凋亡 基因表达 大鼠 

分 类 号：R285[医药卫生—中药学]