三步序贯法对激素干预后哮喘大鼠模型血清IL-4、IFN-γ含量及其肺组织mRNA表达的影响  

Influence of 3-step sequential therapy on serum IL-4, IFN-γ and lung mRNA expression in rat asthma model interfered with hormone

作　　者：崔红生[1] 杨硕[1] 何丰华[1] 武维屏[1] 王硕仁[1] 任传云[1] 

机构地区：[1]北京中医药大学东直门医院,北京100700

出　　处：《北京中医药大学学报》2006年第6期381-384,388,i0001-i0002,共7页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(No.30371714)

摘　　要：目的观察三步序贯法对激素干预后哮喘大鼠模型血清IL-4、IFN-γ含量及其肺组织mRNA表达的影响。方法将SD大鼠随机分为正常对照组、哮喘模型组、激素干预组、三步序贯组、第一步方组、第二步方组、第三步方组、普米克令舒组。制备激素干预后哮喘大鼠模型,于第3周起开始撤减激素用量,按照每周地塞米松0.1mg/kg速度递减,直至第7周完全撤除。采用ELISA法检测血清中IL-4和IFN-γ含量,用原位杂交技术检测各组大鼠肺组织中IL-4mRNA和IFN-γmRNA的表达变化。结果与哮喘模型组和激素干预组比较,三步序贯组IL-4的血中含量和肺组织中mR-NA的表达在各个时段均有显著性降低(P<0.01),而IFN-γ的血中含量和肺组织中mRNA的表达在各个时段均有显著性升高(P<0.01),与普米克令舒组比较,在第2周末,二者无统计学意义(P>0.05);在第7、9周末皆有极显著性差异(P<0.01)。结论三步序贯法可减少激素干预后哮喘大鼠模型IL-4血中含量及其在肺组织中的mRNA表达,提高IFN-γ血中含量及其在肺组织中mR-NA表达,调节IL-4/IFN-γ的失衡,减轻气道炎症的作用。Objective To observe the influence of 3-step sequential therapy on serum interleukin-4 （IL- 4）, IFN-γ and lung mRNA expression in rat asthma model interfered with hormone. Methods To divide SD rats randomly into the normal control group （group 1 ）, asthma model group （group 2 ）, hormone interfered group （ group 3 ）, 3-step sequential therapy group （ group 4）, first-step group （ group 5 ）, second-step group （ group 6）, third-step group （ group 7 ） and Pulmicort Respules group （ group 8 ）. After the establishment of rat asthma model interfered with hormone and on third week the hormone was withdrawn successively at the dose of 0. 1 mg/kg per week, and until 7^th week the hormone was withdrawn completely. The contents of serum IL-4 and IFN-γ were detected with ELISA method, and the expressions of IL-4 mRNA and IFN-γ mRNA in the lung tissue were examined by in situ hybridization in different groups. Results The contents of serum IL-4 and its lung mRNA expression were decreased significantly in different phases （ P 〈 0. 01 ）, and the content of serum IFN-γ and its lung mRNA expression were increased significantly in different phases （P 〈0.01 ） in group 4 compared with that of group 2 and group 3. There was no statistical significance in the comparison between group 4 and group 8 （P〉0. 05 ） at the end of 2nd week, but a great significant difference at the end of 7^th and 92 weeks （P〈0. 01 ）. Conchmion The 3-step sequential therapy can decrease, in rat asthma model interfered with hormone, the content of serum IL-4 and its lung mRNA expression, increase the content of IFN-γ and its lung mRNA expression, regulate the imbalance of IL-4 and IFN-γ , and relieve the inflammation of respiratory tract.

关 键 词：支气管哮喘 地塞米松 IL-4 IFN-Γ 三步序贯法 普米克令舒 大鼠 

分 类 号：R285.5[医药卫生—中药学]