淫羊藿总黄酮注射液对H_2O_2诱导心肌细胞损伤的影响  

Influence of EFI on cardiocyte lesion induced by H_2O_2

作　　者：黄秀兰[1,2] 王伟[3] 张雪静 范晓蓉 周亚伟 

机构地区：[1]中央民族大学 [2]北大世佳研究中心北京100084 [3]北京中医药大学 [4]北大世佳研究中心

出　　处：《北京中医药大学学报》2006年第6期404-407,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家民族事务委员会资助项目(No.05XY07)

摘　　要：目的观察淫羊藿总黄酮注射液(EFI)对H2O2诱导心肌细胞损伤的保护作用。方法通过体外培养大鼠乳鼠心肌细胞,MTT法检测心肌细胞增殖状况,流式细胞仪检测心肌细胞凋亡,透射电镜观察心肌细胞超微结构。结果H2O2可剂量依赖性增加对心肌细胞增殖的抑制率,100μmol/LH2O2作用12h后心肌细胞凋亡率为(23.94±3.52)%,高于正常对照组的(1.98±1·22)%;心肌超微结构呈凋亡特征性变化;100、200、400mg/LEFI可剂量依赖性降低心肌细胞抑制率,显著降低心肌细胞凋亡率,分别为(15.12±3.61)%、(9.38±3.14)%和(4.49±0.52)%;且能改善心肌细胞超微结构变化。结论淫羊藿总黄酮注射液对H2O2诱导的心肌细胞损伤具有保护作用。Objective To observe the protective effect of Epimedium Flavonoids Injection （EFI） on cardiocyte lesion induced by H202. Methods The cardiocytes of neonatal rats were cultured in vitro. The proliferation of cardiocytes was detected by MTI＂ assay, the apoptosis of cardiocytes detected by flow cytometer and the ultrastructure of cardiocytes was observed by the transmission electron microscope （TEM）. Results H202 increased the inhibitory rate of cardiocyte proliferation in a dose-dependent way. The cardiecyte apoptotic rate was （23.94 ± 3.52） % twelve hours after 100 p,mol/L of H202 administration, which was higher than that of the control group [ （ 1.98± 1.22） % ]. The cardiocyte ultrastructure showed the apoptotic changes. EFI in doses of 100, 200 and 400 mg/L decreased the inhibitory rate of cardiocyte proliferation in a dose-dependent way, decreased significantly the cardiocyte apoptotic rate respectively [ （15.12±3.01） %, （9.38 ±3.14） % and （4.49±0.52） % ], and EFI also relieved the change of cardiocyte ultrastructure. Conclusion EFI has a protective effect on cardiocyte lesion induced by H2O2.

关 键 词：淫羊藿总黄酮注射液 心肌细胞 H2O2 凋亡 乳鼠 

分 类 号：R285.5[医药卫生—中药学]