尿纤维连接蛋白检测在膀胱癌患者中的临床应用  

The Clinical Application of Urine Fibronectin Detection in the Patients with Bladder Cancer.

作　　者：阮国祥[1] 范剑[2] 楼滨[2] 

机构地区：[1]温州医学院检验医学院2000年级,浙江温州325035 [2]浙江大学附属一院检验科,浙江杭州31000

出　　处：《检验医学教育》2006年第2期38-40,共3页

摘　　要：目的:探讨尿纤维连接蛋白(Fibronectin,Fn)在膀胱癌患者中的临床应用价值。方法:采用ELISA 方法检测78例膀胱癌患者(组1)、63例其他泌尿生殖系统疾病患者(组2)及88例健康人(对照组,组3)的尿 Fn含量,并同时测定尿肌酐(Creatin,Cr),计算尿Fn/Cr比值。比较各组人群尿Fn和尿Fn/Cr之间的差异, 并分析不同肿瘤分期膀胱患者的尿Fn,尿Fn/Cr比值之间的差异。结果:膀胱癌组尿Fn含量(594.37±990. 72 ug/L)、Fn/Cr比值(192.28±1047.72 mg/mol)与其他泌尿生殖系统疾病组的尿Fn含量(351.63±859.07 ug/L)、尿Fn/Cr比值(65.25±206.35 mg/mol)及对照组的尿Fn含量(173.08±481.02 ug/L)、对照组的尿 Fn/Cr比值21.92±98.73 mg/mol(P<0.05)相比,差异有显著性(P<0.01,P<0.05)。受试者工作曲线(Receiver Operating Characteristic Curve,ROC曲线)显示:尿Fn用于膀胱癌患者临床诊断的灵敏度和特异度分别为77.1%和78.2%,尿Fn/Cr分别为79.2%和83.0%。另外,不同分期的肿瘤患者的尿Fn含量,尿Fn/Cr 比值也有明显差异(P均<0.05)。结论:尿Fn和尿Fn/Cr在膀胱癌患者的临床诊断方面有着重要的应用价值,是诊断膀胱癌的一种较理想的指标,并可用于膀胱癌患者的预后评估。Objectives：To discuss the clinic applied value of urine fibronectin（Fn）in the patients with bladder transitional cell carcinoma. Metheds：Urine Fn was detected by means of ELISA technique in 78 cases of bladder cancer （group 1）, 63 cases of other urogenital diseases （group 2）and 88 cases of healthy subjects （group 3）. Meanwhile we carried out the analysis of creatin（Cr）in urine and calculated the urine Fn/Cr ratio. Analysed the discrepancy of urine Fn and Fn/Cr among three groups and among tumor grades. Results：The urine Fn and Fn/Cr ratio were significantly higher in bladder cancer （Fn 594. 37±990. 72 ug/L, Fn/Cr 192. 28±1047.72 mg/mol） than in other urogenital diseases （Fn 351.63±859.07 ug/L, Fn/Cr 65.25±206. 35 mg/mol） and the healthy subjects（Fn 173.08±481.02 ug/L,Fn/Cr 21.92±98. 73 mg/mol ） （P〈0. 01 ,P〈0. 05=.. Show by Receiver Operating Characteristic Curve：The sensitivity and specificity of urine Fn were 77.1% and 78.2% respectively in the patients with bladder cancer , while urine Fn/Cr ratio were 79.2% and 83. 0% respectively . Otherwise ,urine Fn and Fn/Cr ratio had obvious discrepancy among tumor grades （P〈0. 05 ,P〈0. 05） Conclusions ：Urine Fn and Fn/Cr ratio have significant diagnostic value in the patients with bladder cancer . Urine Fn was claimed to be a optimal marker in the diagnosis and prognosis of bladder cancer.

关 键 词：尿纤维连接蛋白 膀胱癌 受试者工作曲线 

分 类 号：R587.1[医药卫生—内分泌] R737.9[医药卫生—内科学]