菜地和稻田土壤真菌的分离方法比较  

A comparison of methods for collecting fungi from field soil planted with rices and vegetables

作　　者：方羽生[1] 李小妮 黄田福[1] 高永峰[1] 王奕恒[1] 

机构地区：[1]华南农业大学资源环境学院,广东广州510642 [2]广东省植物保护总站,广东广州510500

出　　处：《仲恺农业技术学院学报》2006年第2期53-55,共3页Journal of Zhongkai Agrotechnical College

基　　金：中国科学院鹤山丘陵综合试验站开放站基金(4200-F03044)资助项目

摘　　要：比较了虎红琼脂培养基、酸性PDA培养基、PDA培养基和植物组织诱集法对菜地和稻田土壤真菌的分离效果.结果表明,虎红琼脂培养基和酸性PDA培养基对土壤真菌的分离效果显著优于PDA培养基(α=0.05).分离时土壤悬浮液以10-1和10-2稀释度最佳.采用植物组织诱集法可分离到土壤中的腐霉菌.The preliminary investigation was carried out on fungi collected from field soil planted with vegetables and rices. The common ways were compared for separating fungi from soil including PDA medium, acid PDA medium, Rose Bengal Agar medium and plant tissue bait. The results showed that collection effect of Rose Bengal Agar medium and acid PDA medium were much better than that of PDA medium （α = 0.05）. On these two medium growth of bacteria and actinomyces was restricted but fungi was influenced rarely. The suitable soil suspended liquid concentration was 10^-1 and 10^-2 when separating. Pythium aphanidermatum and Pythium nagaii were collected by plant tissue bait method from rice soil sample.

关 键 词：土壤真菌 分离方法 选择性培养基 植物组织诱集法 

分 类 号：S154.3[农业科学—土壤学]