病毒性心肌病患者淋巴细胞线粒体增生及膜磷脂脱失定量分析  

Quantitative analysis of mitochondrial proliferation and loss of mitochondrial phospholipid membrane localization of lymphocytes in patients with viral myocardial disease

作　　者：魏瑾[1] 陈明霞[2] 耿涛[3] 刘治全[3] 

机构地区：[1]西安交通大学医学院第二附属医院心内科,西安710004 [2]西安交通大学医学院电镜室 [3]西安交通大学医学院第一附属医院心内科

出　　处：《临床心血管病杂志》2006年第7期390-392,共3页Journal of Clinical Cardiology

摘　　要：目的:观察病毒性心肌病患者外周血淋巴细胞线粒体增生及线粒体膜磷脂脱失程度,并分析该损伤与心功能状态的关系。方法:电镜细胞化学法定量观察83例病毒性心肌病患者(病毒性心肌炎50例,扩张型心肌病33例;NYHA心功能Ⅰ级者45例,Ⅱ级者9例,Ⅲ级者16例,Ⅳ级者13例)外周血淋巴细胞线粒体增生及线粒体膜磷脂脱失程度,并按心功能分级进行分析。23例健康献血员外周血淋巴细胞为正常对照组。结果:病毒性心肌病患者外周血淋巴细胞线粒体明显增生,线粒体总数及>10个线粒体淋巴细胞所占比例显著增加,<5个线粒体的淋巴细胞所占比例显著降低;膜磷脂定位正常的线粒体所占比率显著下降,而膜磷脂脱失线粒体所占比率显著升高;心功能Ⅰ级的病毒性心肌病患者外周血淋巴细胞线粒体增生和膜磷脂脱失的程度已显著高于正常对照组,且这些变化随着心功能损伤的加重更为明显。结论:线粒体膜磷脂损伤是病毒性心肌病发生发展的一个重要机制。Objective.. To observe the degrees of mitochondrial proliferation and loss of mitochondrial phospholipid membrane localization of lymphocytes in patients with viral myocardial disease and their relation to cardiac functions. Method： Mitochondrial proliferation and loss of mitochondrial phospholipid membrane localization of lymphocytes in 83 patients with viral myocardial disease（50 with viral myocarditis and 33 with dilated cardiomyopathy） were ＂studied by electron microscope using Dermer＇s tricomplex flocculation technique, the severity of which was analyzed according to NYHA cardiac functions. 23 healthy blood donors were selected as the controls. Result.. The levels of the mitochondrial proliferation and the loss of mitochondrial phospholipid membrane localization were significant in patients with viral myocardial disease compared with those in the controls. The mitochondrial proliferation and the loss of mitochondrial phospholipid membrane localization in patients with normal cardiac function were different from those in controls and showed positive relation to the severity of cardiac function. Conclusion： The impairment of mitochondrial phospholipid membrane localization might play an important role in the pathogenesis of viral myocardial disease.

关 键 词：病毒性心肌病 淋巴细胞 线粒体 膜磷脂 

分 类 号：R542.2[医药卫生—心血管疾病]