原发性高血压内皮一氧化氮合酶基因eNOSG984T变异与高胰岛素血症的关系  

Relationship between eNOS gene G894T mutation and hyperinsulinemia in patients with essential hypertention

作　　者：赵芙[1] 冯少娴[1] 李广镰[1] 马虹[2] 胡斌[3] 

机构地区：[1]广州市第一人民医院心内科,广州510180 [2]广东省中山大学附属第一医院心内科 [3]中山大学达安基因诊断中心

出　　处：《临床心血管病杂志》2006年第7期413-416,共4页Journal of Clinical Cardiology

基　　金：广东省医学科学技术研究基金(No:A2004538)

摘　　要：目的:探讨原发性高血压内皮一氧化氮合酶基因eNOSG894T变异与高胰岛素血症的关系及其临床意义。方法:非糖尿病原发性高血压患者150例,根据空腹胰岛素水平分为非高胰岛素血症、高胰岛素血症及明显高胰岛素血症;正常血压对照组70例。运用聚合酶链反应限制性片段长度多态性检测各组eNOSG894T变异,比较各组eNOSG894T变异基因携带或非携带者的代谢指标。结果:明显高胰岛素血症患者eNOSG894T等位基因频率显著高于正常血压对照组及非高胰岛素血症者(分别为24·6%,10·7%,13·7%,均P<0·01);明显高胰岛素血症患者eNOSG894T变异基因携带者的收缩压、平均动脉压、餐后血糖、三酰甘油及尿酸明显高于正常血压对照组,高密度脂蛋白则明显低于正常血压对照组(均P<0·05)。结论:eNOSG894T变异基因与高胰岛素血症密切相关,可作为高血压患者高危预后评估的指标。Objective：To investigate the clinical implication of the relationship between eNOS gene G894T mutation and hyperinsulinemia in patients with essential hypertension. Method：According to the levels of fasting plas ma insulin , 150 cases with nondiabetic essential hypertension,divided into three groups （nonhyperinsulinemia , hyperinsulinemia and marked hyperinsulinemia） ,were comparded with 70 normahensive controls that divided into noninsulin resistant and insulin resistant groups without/with abnornal clinical metabolic indexes. Polymerase chain reaction and BanⅡ restriction enzyme digestion were performed to detect the eNOSG894T mutation among the groups. Systolic blood pressure（SBP）, mean arterial pressure（MAP）and other metabolic indexes were compared between gene carriers and nongene carriers among the groups. Result： ①The T allele frequency was higher in the marked hyperinsulinemia hypertension group than that in the noninsulin resistant normaltensive controls and the nonhyperinsulinemia hypertension group respectively（24.6%; 10.7% ,24.6% ： 13.7% （ P 〈0.01）. ②SBP, MAP, PBS,TG and UA of eNOSG894T carriers in the marked hyperinsulinemia hypertension group were significantly higher than those in the noninsulin resistant normaltensive controls and the nonhyperinsulinemia hypertension group （P 〈 0.05）, while HDL-C was the lowest among the groups （P 〈 0.05）. Conclusion： Due to eNOSG894T mutation associated with hyperinsulinemia s in essential hypertension patients with abnormal metabolic indexe, it may be a marker for the prognosis of the high risk patients.

关 键 词：高血压 高胰岛素血症 一氧化氮合酶基因eNOS894G 

分 类 号：R544.1[医药卫生—心血管疾病] R587.1[医药卫生—内科学]