无菌容器打开时盖子内面朝向与被污染状况研究  

Study on the relationship between direction of lid's inside and polluted condition when aseptic container opened.

作　　者：马为梅[1] 贾莉[1] 刘灿兰[1] 靳明萍[1] 陈玉琳[1] 王桂明[1] 

机构地区：[1]泰安市中心医院,山东泰安271000

出　　处：《齐鲁护理杂志》2006年第02A期203-204,共2页Journal of Qilu Nursing

摘　　要：目的:通过观察培养皿的细菌生长情况,研究在打开无菌容器时,保持在手中的盖子,内面翻转向上或是仍朝向下,哪种操作更不易被污染。方法:用培养皿模拟无菌容器,皿基在上模拟盖子;打开时,对照组手中的盖子内面翻转向上,实验组则仍朝向下,送细菌培养。结果:两组各160个培养皿中,对照组有54个皿、78个菌落生长;实验组有15个皿、17个菌落生长,两组染菌率和菌落计数差异均有高度显著性(P<0.01)。结论:打开无菌容器时,保持在手中的盖子内面朝向下方更不容易被污染;盖子内面翻转向上者,随暴露时间的延长、环境微生物含量的增多,被污染的可能性增加,盖子内面向下者变化不明显。Objective ： To prove methods which are not easier to be polluted through observing the growth of the colony in culture dish when aseptic container being opened, with the inside of the lid being kept up or down in hand. Methods： Taking the culture dish as aseptic container and the culture medium as the lid. When we opened the culture dish, the inside of the lid was uncovered up in control group while it was uncovered down in experiment group. Results： 160 culture dishes were used in each group. The colony of 78 was found grown in 54 dishes in the control group and the colony of 17 was grown 15 culture dishes in the experiment group. There are significant differences between the two groups in rate of infecting bacteria and the colony amount （P 〈 0.01 ）. Conclusion： When aseptic container opened, the lid is not easier to be polluted when its inside is kept down than up. The lid whose inside is kept up is easy to be polluted with exposed time lengthening and colony growing. But the change is not obvious when its inside is down.

关 键 词：无菌容器 持盖方法 污染程度比较 

分 类 号：R473.5[医药卫生—护理学]