心肌梗死大鼠心脏ACE2基因的表达意义  

Expression of ACE2 mRNA in rat myocardium after acute myocardial infarction and its significance

作　　者：陈高[1] 黄从新[1] 赵庆彦[1] 姚艳[1] 

机构地区：[1]武汉大学人民医院心内科,湖北武汉430060

出　　处：《第四军医大学学报》2006年第13期1202-1204,共3页Journal of the Fourth Military Medical University

基　　金：武汉大学十五211工程建设项目(2003-1684)

摘　　要：目的:研究大鼠ACE2的mRNA在急性心肌梗死(AM I)后大鼠心脏组织中的表达.方法:将36只SD雌性大鼠按时间段随机分为3组(每组12只),每组再随机分为假手术组、手术组2个亚组(每组6只),应用RT-PCR方法检测大鼠AM I后1,3,28 d左心室梗死区及非梗死区心肌ACE2mRNA的表达,并以图像分析系统对其进行半定量分析.结果:在AM I后1 d,与假手术组相比,手术组梗死区及非梗死区ACE2 mRNA水平均无差异(P>0.05).在AM I后3 d,与手术组非梗死区相比,手术组梗死区ACE2 mRNA水平明显升高(P<0.01).在AMI后28 d,与假手术组相比,手术组非梗死区ACE2 mRNA水平也明显升高(P<0.01).结论:AMI后ACE2 mRNA表达增多.AIM： To investigate the changes of the mRNA expression of ACE2 after acute myocardial infarction （ AMI ） in SD female rat. METHODS： Thirty-six SD female rats were randomly separated into 3 groups （n = 12 in each one） according to the infarction duration （ 1,3, 28 d） , which were randomly and evenly again separated into 2 subgroups ： operated group and sham operated group. The levels of mRNA expression of ACE2 in the noninfarct and infract area of left ventricle 1, 3, 28 d aftei AMI were detected by RT-PCR and analyzed semi-quantitatively by image- analysis system. RESULTS ： Compared with sham operated group, the mRNA expression of ACE2 had no significant difference in the infract and noninfarct areas of operated group at day 1 after AMI （ P 〉 0.05 ）. The mRNA expression of ACE2 increased in the infract area compared with the noninfarct area at day3 after AMI （P 〈0.01）. At day 28, increase of ACE2 mRNA expression were also seen in the noninfarct area of AMI rats compared with the myocardium of control rats （P 〈 0.01 ）. CONCLUSION： The gene expression of ACE2 at mRNA level was significantly increased in the myocardium after AMI.

关 键 词：大鼠 ACE2 MRNA 心肌梗死 

分 类 号：R541.4[医药卫生—心血管疾病]