类透明质酸壳聚糖微乳对小鼠血脑屏障通透性的影响  

Effect of hyaluronic acid chitosan-based microemulsion on the permeability of blood brain barrier in mice

作　　者：姚静[1] 周建平[1] 平其能[1] 卢韵[1] 于力[1] 

机构地区：[1]中国药科大学药剂教研室,江苏南京210009

出　　处：《药学学报》2006年第7期615-618,共4页Acta Pharmaceutica Sinica

摘　　要：目的研究类透明质酸壳聚糖微乳(HAC-ME)对血脑屏障开放的促进作用。方法采用甲酰胺提取-紫外分光光度法测定小鼠各脏器中伊文思蓝(EB)的浓度,考察HAC-ME对伊文思蓝在小鼠体内组织分布的影响;荧光显微镜观察脑组织切片中伊文思蓝的荧光强度和分布。结果与未修饰的微乳比较,低浓度的HAC(<5 mg.mL-1)表面修饰微乳可进一步促进伊文思蓝透过血脑屏障,Tm ax延后0.5 h左右,同时降低肝、肾组织中EB浓度。结论HAC-ME可显著增加血脑屏障的通透性,提高药物的脑趋向性,该作用与HAC的浓度有关。Aim To study the effect of hyaluronic acid chitosan-based microemulsion （HAC-ME） on the permeability of blood brain barrier（BBB） by using Evans blue （EB） as the indicator. Methods A formamide extraction-ultraviolet spectrophotometry method was employed to determine the concentrations of EB in each of the tissues. The in vivo distribution of HAC-ME groups containing EB in mice and the fluorescence intensity and diffusion domain of brain slices were all studied. Results Contrasting to the common microemulsion （ME） , HAC-ME at the lower concentration of HAC （ 〈 5 mg 〈 mL^-1 ） could further improve the transporting of EB across the BBB while EB concentration in other tissues decreased, and Tmax was delayed about 30 min. Conclusion HAC-ME could facilitate the transporting of EB across the BBB and it was concentration dependent. While the brain targeting absorptive capability of HAC-ME was enhanced.

关 键 词：类透明质酸壳聚糖微乳 伊文思蓝 血脑屏障 

分 类 号：R943.4[医药卫生—药剂学]